1. The Microscope Mr. Gacc/Bakka BHS Visit www.worldofteaching.com for more free powerpointswww.worldofteaching.com

2. The History Many people experimented with making microscopes Was the microscope originally made by accident? (Most people were creating telescopes) The first microscope was 6 feet long!!! The Greeks & Romans used “lenses” to magnify objects over 1000 years ago.

3. The History Hans and Zacharias Janssen of Holland in the 1590’s created the “first” compound microscope Zacharias Jansen 1588-1631 The “First” Microscope

4. The History Robert Hooke & Anton van Leeuwenhoek made improvements by working on the lenses Anton van Leeuwenhoek 1632-1723 Robert Hooke 1635-1703 Hooke Microscope

5. History In 1665 Robert Hooke examined a thin slice of cork with a single magnifying lens and observed tiny compartments He gave them the latin name cellulae, meaning small rooms - hence the origin of the term cell. He later observed cells in plant tissues and observed the cells were “fill’d with juices”

6. History In the early 1700’s Leeuwenhoek looked at pond water and called the microscopic organisms “animalcules” These were the first observations of living cells 1820 - Robert Brown observed a nucleus in orchid cells

7. History In 1838, Matthius Schleiden stated all plants are made of cells Theodor Schwann stated all animals are made of cells.

8. Cell Theory - 3 parts 1.Cells are the basic unit of life In 1855, Rudolf Virchow further extended the cell theory and added: 2.All organisms are composed of one or more cells 3. All cells come from pre-existing cells

9. How a Microscope Works Convex Lenses are curved glass used to make microscopes (and glasses etc.) Convex Lenses bend light and focus it in one spot.

11. How a Microscope Works Ocular Lens (Magnifies Image) Objective Lens (Gathers light, magnifies and focuses image inside body tube) Body Tube (Image Focuses) Bending Light: The objective (bottom) convex lens magnifies and focuses (bends) the image inside the body tube and the ocular convex (top) lens of a microscope magnifies it (again).

12. The Parts of a Microscope

13. Body Tube 1.The body tube holds the objective lenses and the ocular lens at the proper distance Diagram

14. Nose Piece 2.The Revolving Nose Piece holds the objective lenses and can be turned to increase the magnification Diagram

15. Ocular Lens or Eye lens 3.Magnifies the specimen image 10x Diagram

16. Objective Lenses 4.The Objective Lenses increase magnification from 4x to 10x to 40x. Diagram

17. Stage Clips 5.These 2 clips hold the slide/specimen in place on the stage. Diagram

18. Diaphragm 6.The Diaphragm controls the amount of light on the slide/specimen Turn to let more light in or to make dimmer. Diagram

19. Light Source 7.Projects light upwards through the diaphragm, the specimen and the lenses Some have lights, others have mirrors where you must move the mirror to reflect light Diagram

20. Arm 8.Used to support the microscope when carried. Holds the body tube, revolving nose piece and objective lenses. Diagram

21. Stage 9.Supports the slide/specimen Diagram

22. Coarse Adjustment Knob 10. Moves the stage up and down (quickly) for focusing your image. Never use coarse adjustment under high power!!!!! Diagram

23. Fine Adjustment Knob 11. This knob moves the stage SLIGHTLY to sharpen the image. Diagram

24. Base 12. Supports the microscope Diagram

25. Magnification

26. To determine your magnification…you just multiply the ocular or eye lens by the objective lens Ocular 10x Objective 40x:10 x 40 = 400 Objective Lens have their magnification written on them. Ocular lenses usually magnifies by 10x So the object is 400 times “larger”

27. Hand lens - 5x Stereo microscope - 10 - 60x & 3d image

28. Electron Microscope can focus up 500,000x

29. Tunneling - cell parts/organells 2 types of electron microscopes Scanning - 3D image of RBCs & insects

31. Body TubeTube Nose Piece Objective Lenses Stage Clips Diaphragm Light Source Ocular Lens or eye lens Arm Stage Coarse AdjustmentCoarse Adjustment. Fine Adjustment Base Skip to Magnification Section

32. Using a Microscope Start on the lowest magnification Don’t use the coarse adjustment knob on high magnification…you’ll break the slide!!! Place slide on stage and lock clips Adjust light source (if it’s a mirror…don’t stand in front of it!) Use fine adjustment to focus Use diaghram for light intensity

33. Caring for a Microscope Clean only with a soft cloth/tissue Make sure it’s on a flat surface Don’t bang it Carry it with 2 HANDS…one on the arm and the other on the base

34. Carry a Microscope Correctly

35. References http://www.cerebromente.org.br/n17/history/neurons1_i.htm Google Images http://science.howstuffworks.com/light-microscope1.htm