1. Microscope 02

2. Carrying a Microscope A B

3. Storing The Microscope
Return the lowest power objective in place
Wrap the cord around the base
Return dustcover

4. Cleaning the Microscope
Use lens paper on all glass parts of the microscope.
Clean oil immersion lens with chemicals provided by your instructor

5. Parts of the Microscope
Ocular
Body tube
Nosepiece
Objective lenses (10, 40, 100x)
Arm
Course adjustment knob
Fine adjustment knob
Stage
Stage clips
Aperture
Diaphragm
Light
Base

6. Microscope Parts Ocular Body tube Head Revolving nose piece Objective
Arm
Stage
Diaphragm
Lever to move stage clip
Course adjustment
Fine adjustment
Light source
Base

7. Using the microscope
Always observe using the LOWEST POWER objective first.
Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob.
Focus, and then move to a higher power objective, if needed.
Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE.
Keep both eyes open to reduce eyestrain.
Determine total magnification of the object by multiplying the power of the ocular (10x) the power by the power of the objective.

8. Preparing a slide
Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water.
Place the edge of a coverslip on the slide so that it touches the edge of the water.
Slowly lower the coverslip to prevent the formation of air bubbles.

9. Oil Immersion Lens
100x
40x

10. Animal Cell

11. Plant Cell

12. Cheek Cell

13. Dispose Biological material

14. Dispose Sharps

15. Clean Up

16. Microscope Parts Quiz

17. Microscope Quiz Which objective uses oil?
If your ocular is 10x and your objective is 40x what is the total magnification of your image?
Why do you need a cover slip and how do you avoid an air bubble?
Where do you dispose slides?

18. P. 45 lab manual
sperm
smooth muscle
blood
Simple squamous epithelial

19. Cheek Cell
0.9% saline

20. Cheek Cell Scrape inside cheek Swirl toothpick with cells in saline
Place a small drop methylene blue on saline
saline

21. Cheek Cell Place coverslip Observe under microscope Draw what you see
Clean up

22. Cheek Cell