2. Introduction to the Microscope  Care  Parts  Focusing

3. Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

4. Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage Stage Clips Coarse Focus Fine Focus Base Diaphragm Light

5. Place the Slide on the Microscope Use Stage Clips Click Nosepiece to the lowest (shortest) setting Look into the Eyepiece Use the Coarse Focus

6. Follow steps to focus using low power Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide What can you find on your slide?

7. SAFETY TIP Always wipe the eye piece with an alcohol swab after viewing to disinfect it. Look through the lens WITHOUT your glasses on (if you wear them). Try to look through the lens with BOTH eyes open.

8. Making a Pond Water Slide Place one or two drops with some algae on slide. Carefully place coverslip at a 45 degree angle and touch the liquid. Lower cover slip until it lies flat.

9. Looking through the eyepiece, slowly turn the coarse adjustment knob, moving the lens away until objects come into focus. Move the slide over the stage with knobs or fingers until a specimen of interest is centered. Observe specimens as desired. Then switch to medium or high power. Use the fine adjustment knob to adjust the focus..

10. Pond Water Lab On a piece of paper make drawings of 2 different organisms you see in a drop of pond water. Identify and label them from photos in your textbook. Describe if you think each organism is single-celled or multi-celled. Label any cell parts you can identify.

11. What you might see Filamentous Algae

12. More things you may see Paramecium Perenema Blepherisma

13. Still more you may see Nematode (Round worm)Amoeba