1. The Microscope Prologue: Packet #3

2. Tools & Procedures Microscopes  Simple  Compound Light  Electron Transmission Electron Microscope Scanning Electron Microscope Prologue: Packet #3

4. Body Tube Revolving Nosepiece Focus (4x) Focus (4x) Low Power(10x) High Power(40x) High Power(40x) Eyepiece (10x) Eyepiece (10x) Arm Arm Stage Stage Coarse Adj. Knob Coarse Adj. Knob Fine Adj. Knob Fine Adj. Knob Base Base Iris Diaphragm Iris Diaphragm Stage Clips Stage Clips Light Light Objective Lenses

5. Compound Light Microscope Series of lenses that use light to magnify images (usually 1000x) Can study dead or live organisms, no smaller than 2um. Used in conjunction with chemical stains & fluorescent dyes to enhance images Prologue: Packet #3

6. Electron Microscopes Use beams of electrons to produce images Up to 1000x more detailed than light microscopes Two main types:  SEM: scanning electron microscope Electrons bounce off of outside of object Produces 3-D image  TEM: transmission electron microscope Electrons pass through an object Produces 2-D image Prologue: Packet #3

7. Conditions Needed For Electron Microscope Use 1. Specimen must be looked at within a vacuum 2. Nothing alive can be placed in an electron microscope 3. Specimen must be covered in a fine film of metal

8. Rat Liver Cells Human Cornea T.E.M. Prologue: Packet #3

9. S.E.M S.E.M.