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Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura,

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Presentation on theme: "Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura,"— Presentation transcript:

1 Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors Kenzo Maehashi, Taiji Katsura, Kagan Kerman, Yuzuru Takamura, Kazuhiko Matsumoto, and Eiichi Tamiya Anal. Chem. 2007, 79, 782-787 Jui-Ming (Ryan) Yang

2 Ryan Yang / 03.05.2007 Outline  Background CNT applications CNT biosensor : detection of Immunoglobulin E  Experimental Setup  Result  Conclusion / Consideration

3 Ryan Yang / 03.05.2007 Background  CNT – FET  CNT – Gas Sensor  CNT – Biosensor Protein Sensor DNA Sensor Lin, Y.-M., et al., IEEE Electron Device Lett. (2005) 26, 823 S Meng, et al. Nano Letter (2007) 7 1 45-50 A Modi et al. (2003) Nature 424 171

4 Ryan Yang / 03.05.2007 Background  Goal: Detection of Immunoglobulin E (IgE)  Aptamer (synthetic oligonucleotides)  Monoclonal Antibody against IgE (IgE-mAb)  Debye Length: distance required for screening the surplus charge by mobile carriers present in the material

5 Ryan Yang / 03.05.2007 Experimental Setup  CNT-FET p+ type substrate / 100nm SiO2 source – drain contact: Ti/Au ethanol thermal CVD growth CNT  Custom-Synthesized DNA Aptamer  Immobilized Aptamer onto CNT 1-pyrenebutanoic acid succinimidyl ester with dry dimethylformamide solutions expose to aptamer in PBS overnight deactivate and block the excess reactive groups via ethanolamine D17.4ext: 5’-NH2-GCG CGG GGC ACG TTT ATC CGT CCC TCC TAG TGG CGT GCC CCG CGC-3’

6 Ryan Yang / 03.05.2007 Result  p-type characteristics in blank PBS  The current increased after the IgE- aptamer modification (at the source- drain bias of 0.2 V )  Stability test  Selectivity test Brovie serum albumin (BSA) binding event

7 Ryan Yang / 03.05.2007 Result  Sharp decrease in the source- drain current after introducing IgE, then saturation at lower values  Non-specific binding of IgE can be suppressed  Comparison to IgE-mAb modified CNT-FET

8 Ryan Yang / 03.05.2007 Conclusion  Aptamer modified CNT detects the immunoglobulin E with a good dynamic range, from 250pM to 20 nM  Promising candidate for the development of an integrated, high-throughput, real-time biosensor  Consideration Experimental setup scheme: gate reference Signal target detection limitation  Questions…

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