1. How Cells Reproduce Chapter 8

2. Understanding Cell Division What instructions are necessary for inheritance? How are those instructions duplicated for distribution into daughter cells? By what mechanisms are instructions parceled out to daughter cells?

3. Reproduction Parents produce a new generation of cells or multicelled individuals like themselves Parents must provide daughter cells with hereditary instructions, encoded in DNA, and enough metabolic machinery to start up their own operation

4. Division Mechanisms Eukaryotic organisms –Mitosis –Meiosis Prokaryotic organisms –Prokaryotic fission

5. Roles of Mitosis Multicelled organisms –Growth –Cell replacement Some protistans, fungi, plants, animals –Asexual reproduction

6. Meiosis Functions only in sexual reproduction Precedes the formation of gametes (sperm and eggs) or spores

7. Chromosome A DNA molecule & attached proteins Duplicated in preparation for mitosis

8. Sister Chromatids Each chromosome and its copy stay attached to each other as sister chromatids until late in the nuclear division process Attach at the centromere

9. Nucleosome A nucleosome consists of part of a DNA molecule looped twice around a core of histone proteins

10. Organization of Chromosomes DNA and proteins arranged as cylindrical fiber DNA histone one nucleosome

11. Cell Cycle Cycle starts when a new cell forms During cycle, cell increases in mass and duplicates its chromosomes Cycle ends when the new cell divides

12. Interphase Usually longest part of the cycle Cell increases in mass Number of cytoplasmic components doubles DNA is duplicated

13. Stages of Interphase

14. Control of the Cycle Once S begins, the cycle automatically runs through G2 and mitosis The cycle has a built-in molecular brake in G1 Cancer involves a loss of control over the cycle, malfunction of the “brakes”

15. Stopping the Cycle Some cells normally stop in interphase –Neurons in human brain –Arrested cells do not divide Adverse conditions can stop cycle –Nutrient-deprived amoebas get stuck in interphase

16. Chromosome Number Sum total of chromosomes in a cell Somatic cells –Chromosome number is diploid (2n) –Two of each type of chromosome Gametes –Chromosome number is haploid (n) –One of each chromosome type

17. Human Chromosome Number Diploid chromosome number (n) = 46 Two sets of 23 chromosomes each –One set from father –One set from mother Mitosis produces cells with 46 chromosomes – two of each type

18. Lots of DNA Stretched out, the DNA from one human somatic cell would be more than two meters long A single line of DNA from a salamander cell would extend for ten meters

19. Stages of Mitosis Prophase Metaphase Anaphase Telophase

20. Bipolar Mitotic Spindle Consists of two distinct sets of microtubules –Each set extends from one of the cell poles –Two sets overlap at spindle equator Moves chromosomes during mitosis microtubule of bipolar spindle

21. Mitosis Period of nuclear division Usually followed by cytoplasmic division Four stages: Prophase Metaphase Anaphase Telophase

22. Early Prophase - Mitosis Begins Duplicated chromosomes begin to condense

23. Late Prophase New microtubules are assembled One centriole pair is moved toward opposite pole of spindle Nuclear envelope starts to break up

24. Transition to Metaphase Spindle forms Spindle microtubules become attached to the two sister chromatids of each chromosome

25. Metaphase All chromosomes are lined up at the spindle equator Chromosomes are maximally condensed

26. Anaphase Sister chromatids of each chromosome are pulled apart Once separated, each chromatid is a chromosome

27. Telophase Chromosomes decondense Two nuclear membranes form, one around each set of unduplicated chromosomes

28. Results of Mitosis Two daughter nuclei Each with same chromosome number as parent cell Chromosomes in unduplicated form

29. Cytoplasmic Division Usually occurs between late anaphase and end of telophase Two mechanisms –Cleavage (animals) –Cell plate formation (plants)

30. Animal Cell Division

31. Cell Plate Formation

32. When Control Is Lost Growth and reproduction depend on controls over cell division Checkpoint proteins: –Growth factors invite transcription of genes that help the body grow –Other proteins inhibit cell cycle changes, such as after chromosomal DNA gets damaged When all checkpoint mechanisms for a particular process fail, a cell loses control over its replication cycle An example of this is cancer

33. Neoplasms Are abnormal masses of cells that lost controls over how they grow and divide –Benign – grow slowly and retain surface recognition proteins that keep them in a home tissue (noncancerous) –Malignant – grow and divide abnormally, disrupting surrounding tissues physically and metabolically (cancerous)

34. HeLa Cells Line of human cancer cells that can be grown in culture Descendents of tumor cells from a woman named Henrietta Lacks Lacks died at 31, but her cells continue to live and divide in labs around the world

35. Culturing Cells Growing cells in culture allows researchers to investigate processes and test treatments without danger to patients Most cells cannot be grown in culture