Download presentation
Presentation is loading. Please wait.
Published byDrusilla Boyd Modified over 9 years ago
1
6- Microbial Nutrition & Growth
2
Bacterial growth
4
electron sourcelithotrophs (inorganic) organotrophs (organic)
5
CHNOPS
6
Singlet oxygen Toxic forms of oxygen contains O 2 2- (peroxide anion)
7
Detoxification enzymes Other antioxidants: carotenoids, glutathione, Vit E & C, etc.
8
Micro- aerophiles
9
Nitrogen cycle Fig 26.17
12
acidophiles basophiles/ alkalinophiles Vibrio Ferroplasma neutrophiles Fig. 2.14
13
Halophiles obligate tolerant Barophiles Extremophiles
14
Ecological associations Antagonistic Synergistic Symbiotic Biofilms Quorum sensing
15
Other kinds of samples: food, water, air, soil, etc.
16
Streak plate
17
Pour plate
18
Spread plate
19
Inoculating needle & loop
20
pipet & pipetters
21
micropipette = micropipet = micropipetter Disposable tip
22
tubes Petri plate flask containers
23
For beer
24
agarbroth agar slant deep agar
26
(for Nutrient Broth: just leave out the agar)
27
General medium Selective medium (nutrient agar) (MacConkey agar)
28
Differential medium (MacConkey agar)
29
Differential media (newer)
30
candle jar capnophilics
31
Anaerobic jarGlove box
32
Laminar flow hood (for aerobes)
33
Standard freezer -20 o C Ultra deep freezer -70 to -86 o C Liquid nitrogen -196 o C refrigerator
34
Lyophilization = freeze-drying Other: dessication, crystallization
35
binary fission
36
budding fragmentation
37
Growth curve
38
Plate count using serial dilutions on spread plates Colony counter
40
Cell counter
41
MPN 26 cells /100 ml (from T6.6) Most Probable Number
42
dry weight Indirect methods metabolic activity
43
Turbidity spectrophotometer
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.