1. 2012-iGEM Break Symmetry Southeast university

2. 凌昕彤 周醒驭 陈牧原 高 珅 Team members 周 乐 程 鹏 桂 凯 张 俊 樊雪龙 徐小丹 贾正阳

3.  Technical Background  Design Scheme  Results && Biobricks &&Potential Work Contents  Biosafety &&Human Practice  Experiment

4. Technical Background Create a star! Main Goal : Engineering bacteria colony to automatically break their own symmetry

9. Whole System AHL transduction Light sensor Toggle switch Antisense FtsZ repress division

10. FtsZ mRNA FtsZ asRNA FtsZ gene FtsZ protein silencing transcription translate Tby genetranscription Lux I geneLux I protein SAM AHL luxR gene express luxR protein combine Luxpr promoter promote pcI promoter Lac promoter lacI promoter lacI gene cI genecI protein lacI protein promote inhibit IPTG ultraviolet rays promote Light sensor promoter cI genecI protein promote Light receiver express Red light OmpC express

11. Light Sensor Red Light1 Blue Light2

12. Red Light Light Sensor Cph8

13. Regulatory BLp ycgZycgF ycgE regulated by the ycgF/E system Blue Light Conformation Binding&&Repr ess Expression Blue Light Light Sensor

14. Toggle Switch

15. lacI+pL LuxR LuxI Generating More AHL Activating AHL-Based Quorum Sensing

16. Antisense RNA

17. Verification for Antisense RNA IPTG=0 After 10 hours in 37°C environment IPTG=100IPTG=200

18. Design Scheme  Related Kits:  j5526=r11+b34+mRFP+tt  i13507=b34+mRFP+tt  i13521=tetR+b34+mRFP+tt  j04450=lac+b34+mRFP+tt  r0011=lacipl  r0051=pci  r0040=ptetr  r0063=luxpl(ahl--)  r0062=luxpr(ahl++)  r1051=r0051=pcI  i732820=b34+LacI+tt  j23039=tetR+b34+luxI+tt  i13973=pcI+b34+yfp+tt  k098991=pcI+b34+gfp+tt  p0451=b34+cI+tt  k092400=b34+luxi+tt  i13202=lacipl+b34+luxI+tt  t9002=tetr+b34+luxr+tt+luxpr+b32+gfp+tt  f2620=ptetr+b34+luxr+tt+luxpr  k145270=pro+b34+luxr+tt  j37033=b34+luxr  k091204=pro+b34+luxr+tt  i0462=b34+luxr+tt  k116640=ptetR+b32+luxr+tt  j37015=ptetR+b34+luxr+tt+luxpr+b34+luxI+b34+gfp+tt  K088006=b33+lacI+lac+b34+cI+b32+gfp+tt+pcI+b34+rfp +tt  I763026=ptetR+b34+lacI+tt  I763035=ptetr+b34+lacI+b34+gfp+tt  p0151=b31+cI+tt  p0440=b34+tetr+tt  k145205=ptetr+b32+gfp+tt  K082034=lac+b34+gfp+tt

19. The Simulation Experiment The Micro-Model -Based on Cellular Automaton The Macro-Model -Based on Improved Cellular Automaton Modeling

21. Approximate Bayesian Computation （ ABC ） for parameter inference in gene expression model Modeling 1. Approximate Bayesian computation (ABC) ABC rejection sampler ABC MCMC 2. Gene expression model

24. Modeling

27. Results

28. Verification for Antisense RNA IPTG=0 After 10 hours in 37°C environment IPTG=100IPTG=200

29. others Potential applications Biosensor Cell Printing Signal Transnduction Potential applications

30. Acknowledgements We would like to thank : Academy of Biomedical Engineering for all these support;

31. 数学模型 文档 反义 光敏启动 开关 AHL Others

32. ③ ② ① 该项目大部分由我们自主完成 （除了非天然序列发夹结构外包合 成），技术上不依赖外援。 项目总结 由于缺乏经验，该项目实现效果 不如我们预期，但已部分实现。 下一步我们将完成元件的 标准化和功能验证，以及各种 材料的整理和项目展示工作。