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UMR 1136 INRA/UHP Tree-microbes Interactions “Physiology and functional genomic of transporters” team Fungal pathogen : Melampsora larici-populina Populus.

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Presentation on theme: "UMR 1136 INRA/UHP Tree-microbes Interactions “Physiology and functional genomic of transporters” team Fungal pathogen : Melampsora larici-populina Populus."— Presentation transcript:

1 UMR 1136 INRA/UHP Tree-microbes Interactions “Physiology and functional genomic of transporters” team Fungal pathogen : Melampsora larici-populina Populus trichocarpa

2 Melampsora larici-populina Haustorium formation Adapted from Mengden et al., Biotrophic interfaces Infected leaves At a cytological level

3 Molecular and metabolic dialogue. Entrainment of plant metabolism by phytopathogenic fungus 1.Sensing nitrogen or carbon availability 2.de-repression of specific metabolic pathways (transporters; enzymes) 3.Plant defence responses are taken up through AA permeases. 4.Plant invertase produce depolymerized sugars that both partners can use. - Which role(s) transporters play in molecular dialogue, in exchange of nutrients ? - Which transporteurs are key molecular markers for the interaction? Divon and Fluhr, 2007

4 Toward an overview of Melamspora “transportome”…… © INRA - TCDB: Transport Classification DataBase http://www.tcdb.org/ 550 families -TransportDB http://www.membranetransport.org/ 1.Retrieve proteins from DB 1.Analysis - Blastp - search for IPR, PFAM domains - search by KOG classification And………manual curation (tblastn for some families)

5 Plant pathogensSaprophytes Plant Symbionts Animal pathogens

6 Increased genetic potential for transmembrane channel proteins (water; solute as small carbohydrates (e.g. glycerol), urea, nitrate, CO 2, H 2 O 2, ions)

7 Family specific to plant-pathogen fungus (at least basidiomycetes). ascomycetes???

8 What makes a pathogen an obligate highly specialized pathogen?

9 1. Less ability to transport and likely utilize mineral nitrogen coumpounds.

10 2. an increased genetic potential for peptide uptake...

11 EuGene.00050100|Mellp1: 93456 EuGene.00920002 Mellp1:96051 estExt fgenesh2 pg.C 920006|Mellp1:11... PGTG 17016.2|predicted protein EuGene.00080166|Mellp1: 95942 EuGene.00270125 Mellp1:88574 EuGene.00710021|Mellp1: 94692 estExt fgenesh2 pg.C 20111|Mellp1:115025 EuGene.00020079|Mellp1: 88948 PGTG 03381.2|conserved hypothetical p... YPR194C (Opt2p) Ca 87045975 (OPT6) Ca 87045971 (OPT5) Ca 87045969 (OPT4) Ca 87045965 (OPT3) Ca 87045961| (OPT2) e gw1.10.26.1 Mellp1:34758 PGTG 06690.2|sexual differentiation p... YJL212C (Opt1p) Ca 68475288 (Opt1p) Scpo 19112445 (Isp4p) EuGene.00200025|Mellp1: 87295 EuGene.00120007| Mellp1:84873 PGTG 02841.2|conserved hypothetical p... Ca 87045979 (OPT7) EuGene.00180121|Mellp1 86467 PGTG 14780.2|predicted protein EuGene.00290023| Mellp1:88746 PGTG 17677.2|hypothetical protein EuGene.00230133|Mellp1: 87906 EuGene.00170147|Mellp1: 86271 PGTG 15138.2|predicted protein PGTG 15149.2|predicted protein EuGene.00230137|Mellp1: 87910 fgenesh2 pg.23 156|Mellp1: 107483 PGTG 17794.2|predicted protein estExt fgenesh2 pg.C 890028 Mellp1:11... PGTG 07859.2|predicted protein PGTG 20168.2|predicted protein EuGene.00220005|Mellp1: 87588 PGTG 07855.2|conserved hypothetical p... PGTG 02060.2|predicted protein PGTG 07635.2|predicted protein PGTG 02671.2|predicted protein PGTG 02023.2|predicted protein PGTG 02025.2|predicted protein PGTG 02175.2|predicted protein estExt fgenesh1 pg.C 150177 Mellp1:77... PGTG 10886.2|conserved hypothetical p... ScYgl114wp Ca 87045983 (OPT8) EuGene.00140070 Mellp1:85440 PGTG 13561.2|conserved hypothetical p... Tetrapeptide signature Mlp genes in tandem Mlp genes Phylogenetic analysis of the OPT family NJ method, Poisson correction, 1000 bootstrap replicates (MEGA4)

12 L. bicolor :2 P. chrysosporium:6 C. cinerea: 2 C. neoformans: 0 U. maydis: 2 P.graminis: 14 M.larici populina: 8

13 Phylogenetic analysis of the APC family YAT ACT LAT AAAP NJ method, Poisson correction, 1000 bootstrap replicates (MEGA4)

14

15 Lacbi1:143007 CC1G 01034.1 Af293 66849835 Agp2 YBR132C AGP2 CNAG 05345.1 CNAG 00912.1 NCU07129.1 YOR348C PUT4 En 630383 PutX NCU00721.1 Phchr1:127717 Lacbi1:245329 Lacbi1:308595 Lacbi1:308597 Lacbi1:188015 Hc 74663954 GAP1 UM03325.1 CNAG 06070.1 CNAG 02539.1 estExt Genewise1Plus.C 350029 Mellp1:... Ufa 15485607 AAT1p e gw1.6.48.1 Mellp1:34045 Ufa 1764097 AAT2 e gw1.26.16.1 Mellp1:37139 estExt Genewise1Plus.C 880007 Mellp1:... fgenesh1 pg.C scaffold 136000006 Mell... UM05269.1 NCU04468.1 PENCH 74638092 Mtr UM04186.1 Lacbi1:300838 CC1G 03688.1 Am 4468097 AAT1 Phchr1:135376 CNAG 01118.1 UM06012.1 e gw1.19.49.1 Mellp1:36162 e gw1.19.48.1 Mellp1:36184 Homology P... estExt fgenesh1 kg.C 250027 Mellp1:72... Ufa 44682842 AAT3p EuGene.00360058 Mellp1:90273 Homology... e gw1.36.25.1 Mellp1:38039 e gw1.97.7.1 Mellp1:41039 Lacbi1:182249 CC1G 04288.1 Phchr1:133012 YPL265W DIP5 UM03700.1 CNAG 00552.1 CNAG 00597.1 NCU05168.1 YNL268W LYP1 YNL270C APL1 YEL063C CAN1 Lacbi1:157448 CC1G 02032.1 Phchr1:4184 Af 21627813 LYP Lacbi1:301980 Phchr1:135277 YFL055W AGP3 UM05035.1 Af293 66846710 Gap1 PENCH 41745538 GAP NCU05830.1 NCU02783.1 UM00343.1 NCU03509 Naap1 CC1G 07764.1 Lacbi1:143243 Lacbi1:296817 Gm 62361830 YLL061W MMP1 YPL274W SAM3 YGR191W HIP1 YKR039W GAP1 YOL020W TAT2 YBR069C TAT1 YCL025C AGP1 YDR508C GNP1 YBR068C BAP2 YDR046C BAP3 NCU00648.1 UM03400.1 Phchr1:4672 UM04695.1 fgenesh2 pg.76 38 Mellp1:113062 fgenesh2 pg.76 44 Mellp1:113068 CC1G 11390.1 Phchr1:139236 Lacbi1:185557 CNAG 05952.1 Lacbi1:185515 Lacbi1:252280 SCHPO 15214396 CNAG 05119.1 UM02146.1 NCU05776.1 CNAG 05017.1 CNAG 02455.1 En 74665835 GabA UM02791.1 YNR056C BIO5 YGL077C HNM1 Lacbi1:234289 CC1G 09113.1 Phchr1:122343 Phchr1:27552 CNAG 01535.1 UM02154.1 estExt Genewise1Plus.C 90054 Mellp1:4... NCU00765 AAP2 Lacbi1:191715 Lacbi1:311288 CC1G 02731.1 Phchr1:125336 NCU07175.1 e gw1.68.5.1 Mellp1:40209 e gw1.32.15.1 Mellp1:37702 Lacbi1:244309 Phchr1:133143 YDL210W UGA4 UM03522.1 Phchr1:135275 Phchr1:5862 Phchr1:26909 Lacbi1:142738 Lacbi1:254728 estExt fgenesh2 pg.C 160013 Mellp1:11... YHL036W MUP3 Lacbi1:229402 CC1G 04186.1 Phchr1:37473 UM01762.1 CNAGJEC21 57223589 CNAG 03955.1 estExt fgenesh2 pg.C 100037 Mellp1:11... Af293 66844481 MUP NCU02195.1 CC1G 07574.1 CNAG 05950.1 Lacbi1:249579 Lacbi1:298603 Lacbi1:298666 Phchr1:126209 NCU07754.1 NCU04942.1 YGR055W MUP1 Lacbi1:166470 CC1G 04074.1 CC1G 08094.1 Phchr1:38005 Lacbi1:250926 CC1G 09573.1 EuGene.00120043 Mellp1:84909 Phchr1:134349 NCU08880.1 UM03258.1 CNAG 06541.1 UM02459.1 Phchr:185 UM00056.1 NCU06619.1 AAP1 CNAG 01074.1 CNAG 05685.1 Lacbi1:141811 CC1G 07103.1 Phchr:45006 CNAG 06828.1 estExt Genewise1.C 40004 Mellp1:41988 NCU03783.1 YKL146W AVT3 UM02946.1 YNL101W AVT4 Phchr1:6774 CC1G 03673.1 UM02159.1 gw1.6.234.1 Mellp1:30092 CNAG 05996.1 Lacbi1:247252 CC1G 01897.1 UM02253.1 gw1.87.12.1 Mellp1:24482 CNAG 03988.1 NCU01481.1 YEL064C AVT2 YBL089W AVT5 YER119C AVT6 YIL088C AVT7 estExt fgenesh2 pg.C 120046 Mellp1:11... UM02221.1 CNAG 01904.1 Phchr:137449 Lacbi1:187989 CC1G 00905.1 0.2 Uromyces fabae genes (PIG, permeases) Mlp genes tandem

16 - OPT : example of how evolution has shaped Mlp genome to suit specialized pathogenic lifestyle. - Role of nutrition in the host-pathogen relationship with respect to host restriction and evolution towards obligate pathogenesis Conclusion Small number of transporter-encoding gene models EST support

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