1. Photosynthesis Lab Review

2. Photosynthesis
Plants, algae, and some bacteria convert light energy into chemical energy that is stored in sugars
Chlorophyll is the primary pigment in chloroplasts

3. Plant Pigment Chromatography
Paper chromotography is used to separate a mixture into its component molecules
The different molecules move up the paper at different rates because of differences in:
Solubility, molecular mass, and hydrogen bonding with the paper

4. You separated plant pigments using an organic solvent (acetone)

5. Analysis of Results
Movement of pigment relative to movement of solvent is a constant, Rf (Reference front)
Rf = distance pigment moved/distance solvent moved

6. Photosynthesis—Light Reactions
Light energy excites electrons in plant pigments
Boosts them to a higher energy state
High energy electrons reduce electron acceptors in the thylakoid membrane
Energy is captured in NADPH & ATP

7. DPIP as an Electron Acceptor
You measured rate of electron excitation when light hits chlorophyll
DPIP is an electron acceptor
As DPIP is reduced, it changes from blue to colorless
To measure the color change, you used a spectrophotometer
Measures amount of light absorbed or transmitted by the sample

8. Design of the Experiment

9. Results
As chlorophyll’s electrons are exited, they reduce the DPIP, and the sample becomes more and more pale, and the transmittance increases

10. Cellular Respiration Lab
Occurs in every cell of all living things
Mitochondria
Nutrients (sugars) convert ADP to ATP
ATP used for all cellular activities that require energy
C6H12O6 + 6O > CO2 + 6H2O + ~38 ATP

11. Design of the Experiment
You measured amount of oxygen consumed by peas using a respirometer

12. Design of the Experiment

13. Results
 temp =  respiration
 germination =  respiration