1. Ling V. Sun & Li Wang Institute of Developmental Biology and Molecular Medicine, Fudan University, and Shanghai Center for Bioinformation Technology, China PBmice and MP-PBmice: A database system of PiggyBac (PB) insertional mutations in mice and a subsystem for mapping the mutations onto the mouse genome with large-scale experimental data

2. Transposons and Insertional Mutations Transposons: Mobile Genetic Elements Barbara McClintock chromosome Transposon Gene 基 因 Transposon Mutant Gene Tagged Insertional Mutagenesis Transgenesis Transposon

3. Advantages of Insertional Mutations can produce easily tractable mutations can produce large number of mutants at low cost and high speed

4. PB(PiggyBac): a high efficient transposition system in mammals Moth piggyBac (Cary et al. Virology 1989) Mammal PB system (Ding et al. Cell 2005) + Act- PBase PB[RFP] PBase PB end

5. Traditional Knock-outPB insertional mutagenesis Reduce Cost & Improve Efficiency Mutant 1Mutant 2 Mutant 3 PB Breeding Mutant ES Screen cell lines Micro- injection IVF Molecular cloning PB: an ideal tool for insertional mutagenesis

6. http://www.idmshanghai.cn/PBmice

7. INSERT interface

9. MP-PBmice system (insertional mutations mapping system of PBmice Experimental Procedure Flow production

12. From PBmice to MP-PBmice Integrated information of PB insertional mutations and their characterizations in mice: PBmice (NAR, 2008 vol.36, Http://www.idmshanghai.cn/PBmice) Daily book-keeping and information exchange between experimental groups for the mapping procedure: MP-PBmice

13. Design and implementation User Interfaces Features

14. MP-PBmice Design and implementation User Interface Features

15. Database Tables: the relational data schema Access-control Table Experimental Stage Table Constant Table Status Table

17. Model-View-Controller (MVC) Pattern & Struts-Spring-Hibernate (SSH) Framework

18. MP-PBmice Design and implementation User Interfaces Features

21. viewDetail detailed information page comment box

22. MP-PBmice Design and implementation User Interface Features

23. Strict Access Control

24. Work Flow Control

25. Expandability Suitable for any genome Suitable for any experimental mapping method

26. Future Plan Improve PBmice Mapping Database Add Bioinformatics Tools Needed

27. Acknowledgments Wenwei Yang Min Han Yuan Zhuang Tian Xu Xiaohui Wu Yun Zheng Ke Jin Jigang Yang Yang Zhong Xing Xie Dongsheng Li Ning Gu For collaboration and further contact: Dr. Ling V. Sun (PI): Lingsun@fudan.edu.cn