1. Exam 3 (mean 76%) A70-63 B62-56 C55-49 D48-42 F41-0

2. Today-Ch 13 Announcements – Look for Chapter 12 and 13 quizzes posted this weekend. –Exam 4 covers Chapters 12, 13 and 16 (last exam you can drop. Everyone must take the final exam)

3. Biology 190 Chapter 16 pictures

4. DNA Replication occurs at the “S” stage of mitosis

5. Fig 5.26 Nucleotides U

6. ACCCCAGACCCCAG Fig 16.5 One DNA strand.

7. Fig 16.6 Double Helix ACCCCAGACCCCAG -T -G -T -C

8. Chargaff’s Rule

9. Question 16.1

10. Functions of Proteins Structural Catalytic activity Immunity Regulation Poisons Transport molecules Hormones Fluid and electrolyte balance Sources of energy

11. Fredrick Griffith’s Experiments Polysaccharide Coat

12. Fredrick Griffith’s Results Live R-strain picked up DNA from the S-strain. Contains genes for “smooth coat”.

13. Alfred Hershey and Martha Chase

14. How viruses (phages) infect bacteria

15. Fig 16.4 Hershey and Chase

16. Watson and Crick

17. Rosalind Franklin’s Data (Maurice Wilkins’ Lab)

18. What exactly IS the structure of DNA?

19. Chargaff’s Rule

20. Nucleotide base pairing

21. Fig 16.7 Structure of DNA

22. Nobel Prize- 1962 WilkinsCrickWatson

23. Figure 16.9 Semiconservative Model for DNA Replication Parental strands (template strands) Daughter strands

24. DNA Origin of Replication Bacteria have one origin of replication. Fig 16.12 Eukaryotic organisms have multiple origins of replication.

25. Unwinding DNA Single-stranded Binding Proteins

26. Nucleoside Triphosphate

27. Fig 16.13 DNA Polymerase (Daughter strand) (Parent strand) (Old strand) P

28. Questions 6.3 & 6.4

29. Fig 16.14 Replication Fork Leading Strand 5’ 3’ Lagging Strand 5’ 3’ 5’ 3’ 5’ 3’ Old strands Parent strands Template strands

30. Fig 16.14 DNA Ligase DNA Polymerase III

31. Fig 16.13 DNA polymerase needs a 3’ -OH group

32. Fig 16.16

33. Fig 16.16. Review of Bacterial DNA Replication Helicase SS binding proteins

34. Fig 16.18 DNA shortening and telomeres

35. Mismatch repair 2 Purines = bulge 2 Pyrimidines = dimple TC GA

36. Fig 16.17 Excision Repair

37. Xeroderma Pigmentosa

38. In very, very, vary rare cases mutations have a selective advantage