1. Progress Report on Miniaturized Liquid Chromatography for NeSSI
Scott Gilbert, CPAC Visiting Scholar
Crystal Vision Microsystems LLC
Atofluidic Technologies, LLC
CPAC Fall Sponsors’ Meeting
NeSSI Workshop Nov 9, 2006

2. Confidential - Crystal Vision Microsystems LLC ©2006
Lab-on-chip approach
Simplicity of operation
Reduction of moving parts – no (mechanical) pumps
Robustness
Inexpensive and facile maintenance
Dionex DX 800 Process HPLC
Confidential - Crystal Vision Microsystems LLC ©2006

3. Initial Approach: Using All Glass Chips
Inlet lines
20mm
Column
Detector electrodes
PEEK chip mount
Confidential - Crystal Vision Microsystems LLC ©2006

4. From Discrete Component Setup…
Confidential - Crystal Vision Microsystems LLC ©2006

5. …To New NeSSI Mountable Design
Confidential - Crystal Vision Microsystems LLC ©2006

6. “Split flow” principle for stream sampling
Liters per minute
microliters per minute
nanoliters per minute
Confidential - Crystal Vision Microsystems LLC ©2006

7. Confidential - Crystal Vision Microsystems LLC ©2006
Fluid Flow Strategy
Split (secondary) flow
Main (primary) flow
Confidential - Crystal Vision Microsystems LLC ©2006

8. Quantitation of flow splitting
Measurements on water flowing passively through a 350 mm x 500 mm x 6 cm microchannel on the block, pressure drop ca. 0.1 psi.
Confidential - Crystal Vision Microsystems LLC ©2006

9. Rapid Prototyping Using Quick Photolithographic Method
Inkjet-printed photomask on transparency film
Finished product – glass / polymer chip
Confidential - Crystal Vision Microsystems LLC ©2006

10. Confidential - Crystal Vision Microsystems LLC ©2006
Functional Integration of m-fluidic LC Chip for On-line Sample Pretreatment
micromixer
diluent in
column
mobile phase in
sample in
Confidential - Crystal Vision Microsystems LLC ©2006

11. Liquid Chromatography for NeSSI™
Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies, LLC
Split flow approach to sampling
m-fluidic LC Chip for On-line Sample Pretreatment
Pulsed electrochemical detection (on-chip)
Liters per minute
microliters per minute
micromixer
diluent in
column
mobile phase in
sample in
nanoliters per minute
Confidential - Crystal Vision Microsystems LLC ©2006

12. Back-Mounted Valves for Flow Control
Confidential - Crystal Vision Microsystems LLC ©2006

13. Front Mounted Microfluidic Chip
m-fluidic chip
Confidential - Crystal Vision Microsystems LLC ©2006

14. NeSSI microLC body machined in Ultem®
Confidential - Crystal Vision Microsystems LLC ©2006