1. Education Pays

2. What do you want to be when you grow up? n Many possibilities to use your biological education. u Biologist u Life Scientist u Doctor/Nurse u BioEngineer u Chemical Engineer YOUR DECISION HAS $$$ CONSEQUENCES!

3. Job Growth n Growth rate is ~35%/yr

4. Salary Information n Median Salary for B.S. =$35k/yr n Starting Salary ~$24/yr-$30k/yr

5. Catalase The Enzyme

6. Using Enzymes in Industry

7. Catalase n Liver Enzyme n Reaction u decomposition of peroxide n 2 H 2 O 2 ----> 2 H 2 O + O 2 (g) n Catalase acts as a catalyst u increases the rate of reaction

8. Rate Plot

9. Catalase Enzyme

10. Catalase - Tetramer

11. Catalase Active Site Heam Site

12. Catalase Mechanism n 2 H 2 O 2 2 H 2 O + O 2 (g)

13. Catalase Mechanism

14. k 1 Fast!! k 2 Slow n k 1 = Enzyme-Substrate Association n k 1 Runs at 4x the Speed Limit, u Diffusion Limit F k1 = 4 x 10 8 Liter/(mole sec) n k 2 = Product Kickout from Active Site u Much Slower than the Speed Limit u Speed limit 10 10 1/sec F k 2 ~10 1/sec

15. Rate Plot

16. Hayes’ Data

17. Catalase Mechanism

18. Constant Determination

19. Hayes’ Data

20. Effect of Catalase Concentration

21. Effect of Temperature

22. Effect of pH

23. To Do Biology, you need n Chemistry n Mathematics n Engineering!