1. SSI Binary Gradient Pump Modular Simplicity

2. Ideal Gradient Characteristics Modular design Small foot print Easy Maintenance Compatible with stand alone control & Data systems 1  l to 5ml/min flow range with 1% resolution

3. Integrated Binary Gradient Pump Small footprint Integrates fluidics Simple control Low dead volume High precision

4. Easy Maintenance Modular design Simple seal changes Self flush is standard Pumps are on pull out drawers Each pump is independently serviceable

5. Fluid Path Fully Accessible Easy Set up –Connect inlet lines –Connect line to Injector –Prime pump 200  l Delay Ez Prime in head PPV

6. Simple Front Panel Control Can select and set –Flow –Max pressure limit –Low pressure limit Allows monitoring –Pressure –Flow Start or stop the pump

7. EZ Prime Allows each head to be primed independently Can prime head while other pump is running

8. High Performance Dead volume is minimized 0.001ml/min flow resolution Out gassing effects minimized since mixing is on high pressure side of pump Precise digital control of each solvents flow Use data system interface for control or keypad controller

9. Low Delay Volume 200  l Delay Flow 0.5ml/min step at 3.0 min

10. 460 480 500 520 Millivolts 35.040.045.050.055.0 Minutes 2% Gradient Steps at 0-10%

11. 0 45 90 135 180 Millivolts 0.000.951.902.853.804.755.706.657.608.55 Minutes Fast Gradients at 3 mL/min 100%A for 2 minutes 0-100%B in 1 min, 3 min

12. Linear and Step Binary Gradient Profile, 250  L/min; Two Runs Superimposed 100%A for 2 min; 0-100%B over 25 min. Gradient Accuracy was determined to be 1% absolute (of set value)

13. Easy System Configuration Add a detector Choose a manual injection valve or Autosampler Choose a column Choose your data collection system Begin

14. Wide Variety of Controllable Detectors FluorescenceUV/Vis –Single Channel –Multi Channel –Diode Array Conductivity Evaporative Light Scattering

15. Works With All Analog Detectors Use EZ Chrom SI with 35900E A to D box Use a keypad controller and a strip chart recorder

16. Application Specific Systems Amino Acid Systems Carbamate Systems Clinical assay systems Genetic Variant system Aflatoxin System Glyphosate system