1. Tipsy Neurons Jackie Barlow Joseph Esdin Trish Hartzell Kalyani Maitra Miriam Martin Xia Yang & Jim Burnette

2. NASI Tidbit Educational context 1. sophomores biology majors in a physiology course mid-semester 2. students have knowledge of Basic chemistry, biology Basic molecular interactions Enzyme-substrate 3. students will be expected to have read background information about neurons, synapses, neurotransmitters Kalyani

3. Goals/Objectives: After this class, students will understand… 1. How signals are transmitted by neurons Inhibitory Excitatory 2. Molecular mechanism of chemical perturbation of neuron function The story continues (next lecture) with signal transduction. Kalyani

4. Learning Outcomes By the end of the lesson, students will be able to: -Draw the sequence of events during neuronal transmission -Compare and contrast inhibitory and excitatory synapses -Predict how receptor agonists and antagonists might interfere with neuron signal transmission Kalyani

5. Assessment Formative Draw the sequence of events during neuron transmission Predict – how receptor agonists and antagonists might interfere with neuron signal transmission – the cumulative effect of multiple ligands for neuron receptors Summative Explain how excitation of an inhibitory pathway can lead to change of function Kalyani

6. Good afternoon Trish

7. Sending messages to the brain stimulus output to brain memory, coordination balance, pain, pleasure

8. Trish

9. The brain is made of billions of neurons Joseph

10. Synapse Presynaptic neuron Postsynaptic neuron Neurons form junctions called synapse and communicate by electrical signals Joseph

11. Synapse Presynaptic neuron Postsynaptic neuron Neurotransmitter release Joseph

12. Neural Transmission Neurotransmitter Synaptic cleft Pre Post Joseph

13. Draw the sequence of events during neuron transmission Joseph

14. Physiology of the Synapse Voltage gated calcium channel 1 2 3 4 Neurotransmitter binding to ligand gated channels (receptors) 6 5 7 7 7 Action potential Vesicles mobilization Neurotransmitter release Changes in the postsynaptic neuron Breakdown of the neurotransmitter Joseph

15. If neurotransmitter is inhibitory! 1 2 3 4 6 5 7 7 7 GABA release Activity decreases in the postsynaptic neuron

16. If neurotransmitter is excitatory! 1 2 3 4 6 5 7 7 7 Glutamate release Activity increases in the postsynaptic neuron Joseph

17. Xia

18. What happens after you drink alcohol? Xia

19. Which image represents the effect of alcohol on the balance between inhibition and excitation? ABCABC Xia Inhibition Excitation Inhibition Excitation

20. Alcohol exposure enhances inhibition and decreases excitation Xia GABA Glutamate

21. Miriam Activity

22. GABA

23. Glutamate

24. How alcohol enhances inhibitory actions of GABA neurotransmission Agonist Jackie (+)

25. How alcohol reduces the excitatory actions of glutamate neurotransmission Alcohol (-) Antagonist Jackie

26. Summative Jackie

27. Alcohol + “downers” = ??? Xanax Jackie

28. Thank You!!!