1. Antioxidant and immonomodulatory activities of polysaccharides isolated from Taxillus Chinensis and Uncaria rhyncophylla
Lin Zhang, Narsimha Reddy, Sundar Rao Koyyalamudi and Sang Chul Jeong 1

2. Background Cancer Treatment
According to the World Health Organisation (WHO), cancer killed more than 11.5 millions people around the world in 2030
Treatment
Western Medicine is Expensive
- Not accessible for majority
Traditional Medicine is cost effective
- More research needed 2

3. Background Botanical Polysaccharides Non-toxicity
Minimal Side- effects
Immunomodulatory properties
Anti-tumour actitive
Joeng et al., 2012 J. Med. Food, 15,
Zhang et al., 2012, Int. J. Biol. Maromol (In press) 3

4. Aim and objective Aim : Objectives :
Isolation and measurement of immunomodulatory activities of polysaccharides from Uncaria rhyncophylla and Taxillus chinensis
Objectives :
Factionation of bioactive polysaccharides from U. rhyncophylla and T. chinensis using gel filtration column
Screening these fractions for their immunomodulatory effects 4

5. Crude polysaccharides
Methodology
Hot water extraction (autoclaving) (121oC, 2h)
EtOH precipitation (95%)
(Supernatant:EtOH =1:4 /24h) Centrifugation (10,447xg / 20min)
Centrifugation (10,447xg / 20min)
Gel chromatography Sepharose CL-6B
Phenol-sulfuric method
Medicinal plant
Supernatant
Precipitate
Crude polysaccharides
Different fractions
Joeng et al., 2012 J. Med. Food, 15, 5

6. Results of fractionation of polysaccharide
Uncaria
Taxillus 6

7. The polysaccharide fractions of Taxillus chinensis:
TCP-1
TCP-2
TCP-3
The polysaccharide fractions of Uncaria rhyncophylla:
UC-1
UC-2 7

8. Preliminary characterisation
Chemical composition analysis
- Analysis of mono-saccharides
- Mainly contains Glucose, Fructose, Xylose, Arabinose and Rhamnose 8

9. Immunomodulatory and Antioxidant activities
- Antioxidant assays
-DPPH- free radical test
-Yeast oxidative stress assay
- Immunomodulatory properties
- NO production
- TNF-α production
- Cell viability 9

10. Results: Antioxidant activity
% Yeast cell growth (Water extracts) *
S.NO (1mg/ml)
Molecular Mass (kDa)
DPPH scavenging activity of water extracts (Ascorbate equivalent µM) #
CHP (150 µM)
H2O2 (2mM)
LAH (75 µM)
TCP-1
541
207 ± 4
27.79 ± 2.11
23.02 ± 1.94
TCP-2 18
447 ± 0
30 ± 3.54
39.7 ± 2.11
25.09 ± 1.46
TCP-3 2
662 ± 0
37.5 ± 0
48.21 ± 2.11
32.65 ± 3.89
UC-1
524
245 ± 0
45 ± 3.54
41.17 ± 4.21
22.68 ± 0.49
UC-2  3
305 ± 0
77.5 ± 7.07
70.52 ± 2.11
25.09 ± 0.49 10

11. Results: Immunomodulatory properties (T. chinensis)11

12. Results: Immunomodulatory properties
(U. rhynchophylla)
4/25/2017 12

13. Results of cell viabilities13

14. Conclusion
Polysaccharides of T. chinensis and U. rhyncophylla displayed high immunomodulatory activities
Huge potential of these polysaccharides as natural anti-tumor agents
Further studies 14

15. References
Schepetkin, I.A. & Quinn, M.T. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential, Immunopharmacology 6 (2006)
Jeong, S.C., Koyyalamudi, S.R., Jeong, Y.T., Song, C.H., & Pang, G. (2012). Macrophage Immunomodulating and Antitumor Activities of Polysaccharides Isolated from Agaricus bisporus White Button Mushrooms, Journal of Medicinal Food, 15,
Sun, L., Peng, X., Sun, P., Shi, J., Yuan, X., Zhu, J., Tai, G., & Zhou, Y. (2012). Structural characterization and immunostimulatory activity of a novel linear α- (1→6)-D-glucan isolated from Panax ginseng CA Meyer. Glycoconjugate Journal, 1-8.
Jeong, S.C., Jeong, Y.T., Yang, B.K., Islam, R., Koyyalamudi, S.R., Pang, G., Cho, K.Y., & Song, C.H. (2010). White button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats, Nutrition Research, 30, 49-56 15

16. Acknowledgements
First and foremost, I would like to thank you my supervisor, Dr. Smith, Dr.Reddy and Dr.Rao for their valuable guidance and advice. I also thank Dr. Jeong for helping me in biological experiments. The generous support of technical staff is also acknowledged. 16

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