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Published byDominic Whitehead Modified over 9 years ago
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Membrane permeation is achieved by a variety of mechanisms
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LeuT Na + -dependent pump KcsA K + channel Ca 2+ ATPase pump ClC Cl – /H + exchanger The number of binding sites varies
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Ion binding correlated with protein conformational changes in a K + channel and a Ca 2+ pump.
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The Structure of the Calcium Channel: Basis of Ca 2+ Conduction and Selectivity Sather and McCleskey (2003), Ann Review Phys
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Membrane topology and amino acid sequences that form part of the pore lining for a cardiac L-type Ca channel.
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Quadruple mutants of the EEEE locus lack high-affinity binding of divalent cations.
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Fanciful depiction of hypothesized structural flexibility in the EEEE locus.
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Three Ca 2+ ions (violet balls) bound to the EEEE locus.
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The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity Mackinnon et al.
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potassium channels permeability and specificity homology of structure
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previous research revealing the pore K+ channel signature sequence figure 1
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targeting the subunit figure 4 white yellow mustard green pink red
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revealing the subunit Michael Andrew Meyer, MD From Neuron to Brain (4th Edition, Sinauer Associates) figure 2 A & B
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the subunit grasp: tetrameric figure 3 A & B
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x-ray crystallography Long et al. 2005 figure 3C
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the selectivity / permeability paradox
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complementation and passage minimize length of crossing encourage those who wish to cross figure 5 A & B
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hold their hands and invite them in figure 7 figure 3D
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specific stabilization figure 8C figure 6
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