2. Jocelyn Sickler Ryan Yashinski

4.  Simple structure  Enclosed by a membrane that separates the content of the vacuole from the cytoplasm and helps maintain the pH (slightly acidic) of the cell by being selectively permeable ◦ Called tonoplast in plants  Fluid inside the membrane contains nutrients and waste products  Do not have a specific size or shape  Related to vesicles which are other membrane-bound sacs found within the cell

5.  Structure: ◦ Only one central vacuole ◦ Take up a large amount of space and is formed by the fusion of many membrane vesicles (tonoplast)  Functions: ◦ Storage ◦ The fluid inside the central vacuole, called cell sap, store organic compounds such as proteins and digestive enzymes and inorganic ions, such as potassium and chloride. ◦ Used as a disposal site for by-products that could damage the cell. ◦ Contain pigments that color the cell ◦ Aid in plant growth by absorbing water ◦ Supports the structure of leaves and flowers Other: Only found in plant cells

7.  Structure: Membrane-bound sac that enlarges with the accumulation of fluid  Function: Pump excess water out of the cell  Other: Usually found in freshwater protozoa and metazoans, such as sponges and hydra

8.  Structure: Same as contractile  Function: Digests food  Other: Found in animals

9.  1. What is the pH of vacuoles? Slightly Basic Slightly Acidic Neutral Strongly Acidic

10.  2. Which of the following is not a vacuole? A. Food Vacuole C. Primary Vacuole D. Central Vacuole B. Contractile Vacuole

11.  3. A central vacuole is found in…? A. Animal cells C. Plant cellsD. A, B, and C B. Bacteria

12.  4. What does a contractile vacuole dispose of from the proximity of the cell? A. EnzymesB. Water D. Hydrogen C. Carbon Dioxide

13.  5. What is the fluid within a central vacuole? A. The Juice D. Cell sap B. Water C. Cytoplasm

14. Good Job! Next…

15. F.A.I.L. Try again!

16.  Questions, comments, or otherwise?