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Table S4. Summary of proteins identified by MALDI-TOF-MS Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence.

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Presentation on theme: "Table S4. Summary of proteins identified by MALDI-TOF-MS Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence."— Presentation transcript:

1 Table S4. Summary of proteins identified by MALDI-TOF-MS Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence coverage*** Function**** 1-1.54YP_095533lpg1504pyruvate dehydrogenase E1 component oxidoreductase protein AceE6.09/10103817030%enzyme 2-1.53YP_095533lpg1504pyruvate dehydrogenase E1 component oxidoreductase protein AceE6.09/10103840551%enzyme 32.04YP_095776lpg1750ClpB protein5.49/9579535161%chaperone 42.05YP_095776lpg1750ClpB protein5.49/9579542767%chaperone 52.06YP_095776lpg1750ClpB protein5.49/9579539563%chaperone 63.01YP_096719lpg2714threonyl tRNA synthase6.09/738658833%enzyme 63.01YP_096333lpg2324L-gulono-gamma-lactone oxidase5.99/713719739%enzyme 78.48YP_096333lpg2324L-gulono-gamma-lactone oxidase5.99/7137132358%enzyme 8-2.74YP_096228lpg2216purine NTPase, putative6.24/677448638%enzyme 91.52YP_095398lpg1369chaperone Hsp90 HtpG5.46/7100812231%chaperone 101.9YP_095408lpg1379urocanate hydratase6.48/6109220044%enzyme 112.02YP_096964lpg2971malate dehydrogenase (NAD-linked), malic enzyme6.13/6188614950%enzyme 126.49YP_096964lpg2971malate dehydrogenase (NAD-linked), malic enzyme6.13/6188632776%enzyme 132.24YP_096263lpg2251succinate semialdehyde dehydrogenase5.63/5160724373%enzyme 142.65YP_094292lpg0238glycine betaine aldehyde dehydrogenase6.02/5291118753%enzyme 142.65YP_094527lpg0483hypothetical protein lpg04835.93/5605612250%unknown

2 Table S4. (continued) Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence coverage*** Function**** 152.02YP_094190lpg0136pyruvate kinase II5.82/5167512139%enzyme 162.37YP_095750lpg1723inosine-5'-monophosphate dehydrogenase6.48/5252911648%enzyme 173.97YP_094237lpg0183amine oxidase, flavin containing6.06/5584523050%enzyme 181.63YP_095380lpg1351piperidine-6-carboxylate dehydrogenase6.21/5523420964%enzyme 192.29YP_095380lpg1351piperidine-6-carboxylate dehydrogenase6.21/5523412246%enzyme 202.22YP_095380lpg1351piperidine-6-carboxylate dehydrogenase6.21/5523421759%enzyme 202.22YP_094183lpg0129methylmalonate-semialdehyde dehydrogenase6.26/5524711540%enzyme 211.86YP_094844lpg0809succinyl-diaminopimelate desuccinylase5.57/5246226742%enzyme 221.85YP_095788lpg1762sigma 54-dependent response regulator6.31/5092421558%transcription regulater 232.57YP_094670lpg0634hypothetical protein lpg06345.71/5077532147%unknown 242.57YP_094670lpg0634hypothetical protein lpg06345.71/5077526955%unknown 252.52YP_095626lpg1597thiolase (acetyl-CoA acetyltransferase )6.72/4703634954%enzyme 266.67YP_095020lpg0987membrane protein, Tfp pilus assembly, pilus retraction ATPase PilT6.62/4979719349%motility 272.19YP_095851lpg1825acyl CoA C-acetyltransferase6.46/4118818335%enzyme 282.49YP_095851lpg1825acyl CoA C-acetyltransferase6.46/4118811228%enzyme 291.55YP_094967lpg09333-dehydroquinate synthetase6.5/4093521566%enzyme

3 Table S4. (continued) Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence coverage*** Function**** 301.72YP_095382lpg13533-ketoacyl CoA thiolase (acetyl-CoA acetyltransferase )6.47/4226417737%enzyme 311.73YP_095382lpg13533-ketoacyl CoA thiolase (acetyl-CoA acetyltransferase )6.47/4226443358%enzyme 322.81YP_094526lpg0482endo-1,4 beta-glucanase6.73/4238535955%enzyme 331.58YP_096290lpg22784-hydroxyphenylpyruvate dioxygenase5.97/4053427333%enzyme 341.57YP_094905lpg08703-hydroxyisobutyryl Coenzyme A hydrolase5.66/3882924950%enzyme 352.86YP_094434lpg0390hypothetical protein lpg03906.29/3959721341%unknown 352.86YP_095733lpg1706arginine/ornithine succinyltransferase7.05/395536327%enzyme 362.11YP_096262lpg2250alcohol dehydrogenase, iron containing5.78/4150718740%enzyme 371.78YP_095945lpg1928 indigoidine synthase A-like protein, uncharacterized enzyme involved in pigment biosynthesis 5.79/3354916038%Toxin production 383.74YP_096220lpg2208zinc binding dehydrogenase6.5/3716918442%enzyme 392.67YP_096220lpg2208zinc binding dehydrogenase6.5/3716920833%enzyme 403.71YP_095906lpg1889lipase6.33/355337234%enzyme 413.69YP_095785lpg1759flagellar motor switch protein FliG4.97/3665214439%motility 421.84YP_094982lpg09482-oxoglutarate ferredoxin oxidoreductase beta subunit8.39/3702315348%enzyme 436.1YP_095906lpg1889 lipase6.33/3553327285%enzyme

4 Table S4. (continued) Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence coverage*** Function**** 441.58YP_096944lpg2951cystathionine beta synthase5.76/3433617857%enzyme 451.77YP_094875lpg0840polysialic acid capsule expression protein6.2/3432425448%enzyme 462.22YP_096868lpg2874hypothetical protein lpg28745.37/3347427661%unknown 471.79YP_096868lpg2874hypothetical protein lpg28745.37/3347410917%unknown 481.63YP_095856lpg1830hydroxymethylglutaryl-CoA lyase5.7/3273228250%enzyme 491.87YP_096618lpg2613UDP-N-acetylenolpyruvoylglucosamine reductase6.46/3396030358%enzyme 501.51YP_095445lpg1416purine nucleoside phosphorylase II5.7/3210415029%enzyme 51-1.68YP_095154lpg1121hypothetical protein lpg11214.69/2872414841%unknown 521.57YP_095751lpg1724septum site-determining protein MinD5.78/3012018431%motility 531.69YP_094561lpg0517aldo/keto reductases, related to diketogulonate reductase6.35/32319839%enzyme 547.5YP_096683lpg2678Hypothetical protein lpg26785.85/3020615355%unknown 557.05YP_094708lpg0672acetoacetate decarboxylase ADC5.43/2832216972%enzyme 5623.36YP_095025lpg0992hypothetical protein lpg09929.46/3172519266%unknown 571.68YP_096690lpg2685dienelactone hydrolase family protein6.38/2744810739%enzyme 571.68YP_094574lpg0531 succinate dehydrogenase iron-sulfur protein subunit B (succinate dehydrogenase catalytic subunit) 6.18/273736852%enzyme

5 Table S4. (continued) Spot no.* Average Ratio** NCBI accesion no. Gene name Protein name Theoretical pI/MW SCORE Sequence coverage** * Function**** 581.71YP_094904lpg08693-hydroxyisobutyryl Coenzyme A hydrolase5.5/2761627936%enzyme 593.01YP_095877lpg1851hypothetical protein lpg18517.85/2535528662%unknown 601.78YP_095877lpg1851hypothetical protein lpg18517.85/253555921%unknown 613.44YP_094904lpg08693-hydroxyisobutyryl Coenzyme A hydrolase5.5/276168516%enzyme 621.79YP_095322lpg1292DNA-binding response regulator5.3/2516026656%transcription regulater 6312.01YP_094601lpg0560acetyoacetyl CoA reductase5.91/2696425564%enzyme 642.03YP_096255lpg2243uracil phosphoribosyltransferase6.19/2387817660%enzyme 651.84YP_094620lpg0584hypothetical phosphate transport regulator5.74/2542326079%unknown 6611.43YP_095092lpg1059acetoacetyl CoA reductase7.53/2929717356%enzyme 671.85YP_096287lpg2275hypothetical protein lpg22756.19/2634916132%unknown 681.66YP_094827lpg0791macrophage infectivity potentiator (Mip)9.19/2514327549%Toxin production 695.07YP_094942lpg0908flagella basal body P-ring formation protein FlgA8.22/260937937%motility 701.65YP_094827lpg0791macrophage infectivity potentiator (Mip)9.19/2514324344%Toxin production 713.93YP_094602lpg0561acetyoacetyl CoA reductase5.85/2737222650%enzyme 728.42YP_094935lpg0901hypothetical protein lpg09016.41/2530915177%unknown 732.94YP_094297lpg0243short chain dehydrogenase6.12/211709038%enzyme

6 Table S4. (continued) Spot no.* Average Ratio** NCBI accesion no. Gene nameProtein name Theoretical pI/MW SCORE Sequence coverage*** Function**** 741.71YP_095993lpg1977intracellular protease, ThiJ/PfpI family5.55/2202119067%enzyme 758.23YP_096447lpg2439NADPH-dependent FMN reductase domain protein7.85/2056023566%enzyme 766.62YP_095674lpg1647hypothetical protein lpg16479.06/2234912056%unknown 771.51YP_096170lpg2157Sid related protein-like (SDeA)5.88/1696397819% substrate of Icm/Dot 771.51YP_096166lpg2153Sid related protein-like (SDeC)5.95/1722626423% substrate of Icm/Dot 781.97YP_096166lpg2153Sid related protein-like (SDeC)5.95/17226225336% substrate of Icm/Dot 791.98YP_096166lpg2153Sid related protein-like (SDeC)5.95/17226215724% substrate of Icm/Dot 80-2.21YP_094288lpg0234SidE5.95/17154411821% substrate of Icm/Dot 81-2.5YP_094288lpg0234SidE5.95/17154410222% substrate of Icm/Dot 82-1.71YP_094288lpg0234SidE5.95/1715446715% substrate of Icm/Dot *Spot No. indicate the circle numbers in 2D-DIGE gel image (Fig. 1). **Ratio means the ratio of the protein spot intensity at PE to that at E; PE/E. ***Sequence coverage means the ratio of the number of identified amino acid sequence to that of the actual whole amino acid sequence. ****Function was assigned based on functions indicated in NCBInr.

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