1. Epithelium and Junctions VIBS 443/602

4. EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells 1.Plasma membrane 2.Basal lamina 3.Connective tissue

5. EM 17; capillary in the lamina propria of the intestine (duodenum); 13,500x 1.Smooth muscle cell 2.Capillary 3.Endothelial cell

6. Renin granules in Kidney (PAS)

7. Ileum, monkey (PAS) 249

8. Trachea, monkey

9. Esophagus and trachea, monkey – glands in trachea

10. Larynx (Gallego's stain)

12. SPECIALIZATION OF EPITHELIA MAINTAIN EXTENSIVE CONTACTS AMONG CELLS STRUCTURALLY AND FUNCTIONALLY POLARIZED JUNCTIONS ZONULA OCCLUDENS - TIGHT JUNCTION (BELT) ZONULA ADHERENS – ADHERING BELT DESMOSOME (MACULA ADHERENS) - SPOT ATTACHMENT GAP JUNCTIONS - COMMUNICATION

13. Terminal bars

14. 32409

15. EM 6b: Region showing zonula occludens, zonula adherents, and macula adherents junctions between cells. 1.Zonula occludens 2.Zonula adherens 3.Desmosome EM 6b

17. EM 6c

18. EM 4: Continuity between junctional complex and terminal web 1.Terminal web 2.Interdigitation of plasma membrane 3.Terminal bar

19. EM 4a: Ridges and grooves of zonula occludens visualized by freeze cleave microscopy 1. Zonula occludens

21. Inside the cell Outside the cell P = Protoplasmic side E = Ectoplasmic side

22. EM 4a

23. EM 2a: Particles and pits of gap junction visualized by freeze cleave microscopy 1. Gap junction

24. EM 2a

25. Triad with bile duct Liver & spleen with colloidal carbon, rat 118

26. EM 2: portion of two cells including gap junction 1.Desmosome 2.Gap junction 3.Rough endoplasmic reticulum

27. absorptive cells of Ileum, monkey 250

28. Ileum, monkey (PAS)

29. argentaffin cells of Ileum, monkey

30. Large intestine or Colon, monkey

31. EM 4c: intestinal absorptive cell (super nuclear region); 60,000x 1.Budding RER 2.Coated vesicle 3.Golgi 4.Mitochondria 5.Nucleus 6.Plasma membrane 7.Primary lysosome

32. Endothelial cells lining small blood vessel lumen with smooth muscle cells and fibrolasts in the vessel wall. 1.Lumen 2.Endothelial cell 3.Fibrolast

33. EM 10a: Endothelial cells lining capillary with pericyte in the vessel wall. 1.Lumen 2.Endothelial cell 3.Tight junction

34. Skeletal muscle in spermatic cord UT 196 196

35. UT196 valves in large vein of spermatic cord 195

36. EM 10f: arteriolar wall; 30,000x 1.Apical caeolae 2.Collagen 3.Dense bodies 4.Elastic fiber 5.Endothelial cell 6.Nuclear pore 7.RER

37. EM 8h: macrophage in testis; 30,000x. 1.Enlarged basal lamina 2.Heterophagic vacuole 3.Leydig cell cytoplasm 4.Nucleolus 5.Myoid cell

38. Skin abdomen

39. Skin, scalp

40. Skin hand monkey 109

41. Skin hand monkey

42. Skin 111

43. Skin, foot

44. Vagina

47. EM 8g: epidermis; 7,000x. Note desmosomal connections between cells 1.Keratinocyte 2.Desmosome 3.Melanocyte

48. EM 16: surface mucosa of stomach; 13,500x 1.Granules of surface mucous cells 2.Mitochondria 3.Nuclei 4.Lumen

49. Urinary bladder, monkey 160

50. Ureter – transitional epithelium and smooth muscle 262

51. Penis – transitional epithelium and surrounding spongy cavernous of penal urethra 277

52. Penis - transitional epithelium of penal urethra 277