1. ‘Scopes & Scientists Review
Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.

2. BIOGENESIS (life comes from life)
ARISTOTLE
ANTON van LEEUWENHOEK
Special “vital” forces brought some living things into being, from nonliving material- “spontaneous generation.”
Discovered microscopic life in pond water.
“Animalcules”- tiny animals
FRANCESCO REDI
BIOGENESIS (life comes from life)
Experiment: covered jars of meat vs. uncovered jars of meat.
Results supported his hypothesis, maggots came from flies.

3. NEEDham = NEEDed to fix his experiment
JOHN NEEDHAM
NEEDham = NEEDed to fix his experiment
Tried to prove spontaneous generation.
Flaw with his experiment: the flask was not sealed and was exposed to the air.
LAZZARO SPALLANZANI
Boiled flasks of gravy to kill life, sealed one jar, left other jar open.
Open jar had living microorganisms, sealed jar did not.
Concluded that gravy did not produce life, organisms entered through the air.

4. spontaneous generation was incorrect.
LOUIS PASTEUR
Designed a flask that had a long curved neck open to air, but microorganisms could not travel through neck to the flask.
Once neck of flask was broken, broth filled with microorganisms.
Showed living things came from living things;
spontaneous generation was incorrect.

5. Light Microscope Stereoscope
Types of Microscopes
Light Microscope
 Uses compound lenses to magnify objects. The lenses bend or refract light to make the object beneath them appear closer.
Stereoscope 
This microscope allows for binocular (two eyes) viewing of larger (or thicker) specimens. Creates a 3D view of specimen

6. Scanning Electron Microscope (SEM)
SEM creates a 3D view of specimen by scanning the surface, but cannot view living specimens (process kills them)
SEM = “surface/scanning”
Transmission Electron Microscope (TEM)
Also uses electrons, but instead of scanning the surface (as with SEMs) electrons are passed through very thin specimens.
TEM = “thin/through”

7. What kind of microscope is this? Compound Light Microscope
This diagram is different than the one you need to label in your packet. Look at where the line is pointing to before you label it in your review packet!
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Red: Scanning
Yellow: Low
Blue: High
Stage
Stage Clips
Coarse Adjustment Knob (BIG one)
Diaphragm
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Fine Adjustment Knob (small one)
Light
Base
What kind of microscope is this?
Compound Light Microscope