1. Inhibitory Gli3 Activity Negatively Regulates Wnt/  -Catenin Signaling Ulloa F. et al, Current Biology (2007)

2. Hedgehog Signaling Varjosalo M. and Taipale J. JCS (2007) Development –Nervous System Disease –Cyclopia –Holoprosencephaly Cancer Homologes of Hedgehog –Sonic hedgehog –Indian hedgehog –Desert hedgehog

3. Shh Wnt1 Fuccillo et al. Nature Reviews Neuroscience (2006) Sonic Hedgehog in the Neural Tube Ventral Dorsal Proliferation and Survival of Neural Progenitors

4. Failure of a medulloblastoma-derived mutant of SUFU to suppress WNT signaling. - Taylor MD. et al, Oncogene (2004) Shifted, the Drosophila ortholog of Wnt inhibitory factor-1, controls the distribution and movement of Hedgehog. - Glise B. et al, Dev Cell (2005) Suppressing Wnt signaling by the hedgehog pathway through sFRP-1. - He J. et al, JBC (2006) Wnt signaling inhibitors regulate the transcriptional response to morphogenetic Shh-Gli signaling in the neural tube. - Lei O. et al, Dev Cell (2006) Gli1 acts through Snail and E-cadherin to promote nuclear signaling by beta-catenin. - Li X. et al, Oncogene (2007) Hedgehog and Wnt Signaling

5. Wnt signaling is decreased in the absence of Shh signaling In Shh null embryos, apoptotic cells were detected in ventral and dorsal regions. Ptc1Δloop2 – a dominant active form inhibits Hh signaling

6. Gli3R Iinhibits Canonical Wnt Signaling

7. Gli3R Inhibits  -Catenin Activity

8. Gli3R Inhibits  -Catenin-Induced Secondary Axis Formation in Xenopus Embryos

9. Gli3R Interacts with  -Catenin

11. Gli3R In the absence of Shh signaling, high levels of Gli3R act to inhibit Wnt signaling. Result sFRP-1(?)