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( Ⅱ ) 臨床生化 ( Ⅱ ) Instrumentational Analysis 賴滄海 教授 10-23-2007.

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Presentation on theme: "( Ⅱ ) 臨床生化 ( Ⅱ ) Instrumentational Analysis 賴滄海 教授 10-23-2007."— Presentation transcript:

1 ( Ⅱ ) 臨床生化 ( Ⅱ ) Instrumentational Analysis 賴滄海 教授 10-23-2007

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4 Individual Steps In the Analytical Processes Specimen acquisition Specimen identification Specimen delivery to laboratory Specimen preparation Specimen loading and aspiration On-analyzer specimen delivery

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20 Ka = 【 Hp -Ab 】 【 Hp 】【 Ab 】 Ka = affinity constant

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24 免疫檢驗的分類 方式 Homogeneous Heterogeneous 原理 Competitive Sandwich 標誌物質 RIA FIA EIA CLIA

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30 Examples of IAs Radio immunoassay (RIA) Particle –enhanced turbidimetric inhibition IA (PETINIA) Enzyme-linked Immunosorbent Assays (ELISA) Enzyme multiplied Immunoassay Technique (EMIT) Cloned enzyme donor IA (CEDIA) Microparticle capture enzyme IA (M EIA) Solid phase Fluorescence IA (SPFIA) Particle concentration Fluorescence IA (PCFIA) Fluorescence excitation transfer IA (FETI) Substrate-level Fluorescence IA (FPIA) Dissociation-enhanced lanthanide fluoro imnurco assay (DELFIA)

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