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Attack of the Superbugs Lab Introduction Mrs. Stewart Medical Interventions
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Superbugs What is a “superbug”? – Bacteria that have become stronger and less responsive to antibiotic treatment
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How can bacteria share resistance? Genetic Transfer Method Description ConjugationThe one-way transfer of DNA (plasmid) between bacteria in close cellular contact TransformationThe genetic modification of a bacterium by incorporation of free DNA from the surrounding environment (usually caused by another ruptured bacterial cell) TransductionThe transfer of genetic material from one bacterium to another by a genetic vector (bacteriophage virus)
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This lab uses 2 types of Bacteria: E. coli I – contains a gene found on the chromosomal DNA coding for streptomycin resistance E. coli II – contains a gene found on the plasmid DNA coding for ampicillin resistance.
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Predict What will happen when the E. coli I strain is mixed with the E. coli II strain?
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Lab Materials: 4 growth plates LB Agar LB + Amp LB + Str LB /Str /Amp *LB Agar = growth medium for bacterial cells
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Lab Day 1: Confirmation Plates Obtain 1 of each type of growth plate – 4 plates total Streak E. coli I on 4 plates Streak E. coli II on 4 plates Incubate overnight
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How to label the plates I II Group Name
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Evaluate What are we confirming? These plates will confirm that our strains of E. coli are correctly labeled
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Data Table E. coli IE. coli II LB agar LB + Str LB + Amp LB + Str + Amp + = Growth/ -- = No Growth
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Prediction Predict the results from your confirmation plates that you will expect to record
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Lab Day 2: Observe confirmation plate results Prepare “mix” plate – You will literally be mixing the two types of bacteria together onto one plate Incubate overnight
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Evaluate Specifically, what will happen when we mix them together?
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Lab Day 3: Use new “mixed” bacterial cultures and spread onto the 3 types of antibiotic plates
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Predict What would you expect to see on these growth plates? Why?
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Lab Day 4 Observe and record your results Remember: Pictures of lab results will be required in your lab reports *Growth plates will be discarded after today. Any results not recorded will be lost.
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Student Lab Schedule Day:Time Needed: Activity:How to Store: Lab Day 1 ~ 30 minutes Students prepare confirmation plates. Invert the plates, label, and incubate at 37˚C for 24 hours. Lab Day 2 ~ 30 minutes Students observe confirmation plates and prepare “Mix Plate.” Invert the plates, label, and incubate at 37˚C for 24 hours. Lab Day 3 ~ 30 minutes Students streak cultures from “Mix Plate” onto antibiotic plates. Invert the plates, label, and incubate at 37˚C for 24 hours. Lab Day 4 ~ 10 minutes Students observe & record results Clean-up all materials.
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