1. 1 International Federation for Consulting GmbH IFC Method Validation and QC for Pesticide Residue Analysis SANCO/2007/3131 by Dr. Michael Scheutwinkel

2. 2 Outline - This SANCO paper applies for all governmental laboratories and those private ones working in the official food control. - Accreditation Bodies are using this paper as basis for validation and quality control. SANCO/2007/3131

3. 3 Calibration…. -representative analytes for each bach of analyses -all substances at least every 3 months At least 15 analytes + 25% of total number of analytes (100 analytes = 40 components) At least 15 analytes + 25% of total number of analytes (100 analytes = 40 components) SANCO/2007/3131

4. 4 Calibration…. -Matrix matched … - Grouping of food stuff … The most effective way to negate matrix effects is to calibrate with standard addition. The most effective way to negate matrix effects is to calibrate with standard addition. SANCO/2007/3131

5. 5 Validation Model List of all pesticides analysed in routine List of all pesticides analysed in routine Repeatability Recoveries, 70 – 120 % Recoveries, 70 – 120 % LOD‘s /LOQ in different types of matrix reproducibiity LOD‘s /LOQ in different types of matrix reproducibiity Reproducibility Pesticide concept Which pesticide is detected how? Which pesticide is detected how? Fulfilment of legal requirements Fulfilment of legal requirements