1. © University of Sydney Australian Cereal Rust Control Program Professor Robert F. Park robert.park@sydney.edu.au Australian Cereal Rust Control Program Consultative Committee DAFWA, Perth March 21 st 2013 Director’s Report

2. © University of Sydney WA residents told to prepare for Cyclone Rusty! Monday 25 th February 2013, AEST 9.00 am http://www.abc.net.au/news/2013-02-25/wa-residents-told-to-prepare-for-cyclone-rusty/4537056

3. © University of Sydney Scientific staff: Urmil Bansal Harbans Bariana Robert Park Peng Zhang Davinder Singh Haydar Karaoglu Peter Dracatos Honorary Associates: William Cuddy Bob McIntosh Ray Hare Colin Wellings General staff: Muhammed Gill Sami Hoxa Keshab Kandel Hanif Miah Gary Standen Matthew Williams Personnel, as @ March 2013 Postgraduate students: Jordan Bailey Mumta Chettri Lida Derevnina Banchgize Temesgen Leila Kamino Tolera Keno Mandeep Randhawa Huda Elmansour Peace Kankwatsa Vallence Nsabesyera Mesfin Gesesse Bosco Chemeyek Ali Salman

4. © University of Sydney Personnel changes during 2012 Four technical positions made redundant: - Mr Gary Standen – Fee for Service, GRDC funded. - Mr Paul Kavanagh – Non-wheat rust surveys and genetics - Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests) - Mr Keshab Kandel – Stripe rust surveys and genetics Dr Wellings retired, June 2012 Park Appointment New Appointments: - Mr Gary Standen appointed, FFS (non-GRDC funds) - Mr Keshab Kandel appointed, Rust surveys - Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases - Business Manager TBA -HEO 3 Technician TBA

5. © University of Sydney Personnel changes during 2012 Four technical positions made redundant: - Mr Gary Standen – Fee for Service, GRDC funded. - Mr Paul Kavanagh – Non-wheat rust surveys and genetics - Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests) - Mr Keshab Kandel – Stripe rust surveys and genetics Dr Wellings retired, June 2012 Park Appointment New Appointments: - Mr Gary Standen appointed, FFS (non-GRDC funds) - Mr Keshab Kandel appointed, Rust surveys - Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases - Business Manager TBA -HEO 3 Technician TBA

6. © University of Sydney Personnel changes during 2012 Four technical positions made redundant: - Mr Gary Standen – Fee for Service, GRDC funded. - Mr Paul Kavanagh – Non-wheat rust surveys and genetics - Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests) - Mr Keshab Kandel – Stripe rust surveys and genetics Dr Wellings retired, June 2012 New Appointments: - Mr Gary Standen appointed, FFS (non-GRDC funds) - Mr Keshab Kandel appointed, Rust surveys - Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases - Business Manager TBA - HEO 3 Technician TBA

7. © University of Sydney Previously, ACRCP was 1 project – now 4 projects: 1. ACRCP - National breeding support. US00064 2. ACRCP - Durable genes. US00063 3. ACRCP - Towards 2019 and a century of monitoring cereal rust pathogens in Australia. US00067 4. ACRCP- Molecular marker program CSIRO/Univ. Sydney/CIMMYT collaborative project. CSP00164 Current status of “new” projects [1 July 2012 thru 30 June 2017] Proposals include: - reducing Director’s salary to zero, incrementally, over project life - GRDC to fund (50%) Business Manager - Fee for Service subsidy to reduce to zero (wheat by year 3, barley by year 4) - full review of FFS, including costings, once Business Manager is appointed - establishment of a Breeder’s Advisory Group

8. © University of Sydney Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007 Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….) Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL. Defence Trade Controls Act 2012

9. © University of Sydney Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007 Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….) Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL. Defence Trade Controls Act 2012

10. © University of Sydney Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007 Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….) Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL. Defence Trade Controls Act 2012

11. © University of Sydney Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007 Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….) Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL. Defence Trade Controls Act 2012

12. © University of Sydney Camden Campus - Centre for Carbon, Food & Water

13. © University of Sydney Camden Campus – CER complex

14. © University of Sydney PBI Infrastructure – greenhouse polycarbonate

15. © University of Sydney Fee for Service, wheat test numbers 2007 through 2012 Wheat:Year Test:200720082009201020112012 Field tests121,08280,642100,37496,81160,33845,076 Greenhouse tests41,62615,9038,28721,99933,20122,509 F 2 Long Rows217295277944611199 Non-wheat:Year Test:200720082009201020112012 Field tests10,62812,21013,14211,6179,88513,128 Greenhouse tests12,51813,72615,02117,32724,99330,128

16. © University of Sydney Fee for Service, wheat test numbers 2007 through 2012 Wheat:Year Test:200720082009201020112012 Field tests121,08280,642100,37496,81160,33845,076 Greenhouse tests41,62615,9038,28721,99933,20122,509 F 2 Long Rows217295277944611199 Non-wheat:Year Test:200720082009201020112012 Field tests10,62812,21013,14211,6179,88513,128 Greenhouse tests12,51813,72615,02117,32724,99330,128

17. © University of Sydney Field rust inoculations in 2012 - 19 pathotypes across 5 field sites CropRustPathotype WheatLeaf rust104-1,2,3,(6),(7),11+Lr37 [=576] 104-1,2,3,(6),(7),11,13 [=547] 10-1,3,7,9,10,12 [=592] 76-1,3,5,7,9,10,12 +Lr37 [=621] Stem rust98-1,2,3,5,6,7 [=580] 34-2,12,13 [=427] 34-1,2,7 +Sr38 [=565] 343-1,2,3,4,5,6 [=429] Stripe rust134 E16 A+ Yr17+ Yr27+ [=617] 134 E16 A+ J+ T+ [=???] 150 E16 A+ [=598] BarleyLeaf rust5457P+ [=612] Stripe rustBGYR OatsCrown rust4473-4,16,10 +BB [=567] 0107-4,6,10+WN [=595] 0207-1,5,6,10,12+G [=549] 0307-345610+WV [=605] 264 [=164] Stem rust41+Pg9,13,a [=512] TriticaleStem rust34-2,12,13 [=427] Stripe rust134 E16 A+ J+ Yr27+ 134 E16 A+ J+ T+ [=???]

18. © University of Sydney Field rust pathotypes – FFS sampling for pathotype ID

19. © University of Sydney Field sites Lansdowne3 Year rotation, 4.5ha Horseunit3 Year rotation, 3.6ha Breakwell3 Year rotation, 1ha Karalee3 Year rotation, 0.7ha J.B. Pye*3 Year rotation, 0.8ha Sheep Unit3 Year rotation, 1.2ha * Also have a further 7.5ha (2.5ha x 3 year rotation) available from 2013

20. © University of Sydney Field – Karalee redevelopment

21. © University of Sydney Dam Roadway “Cage 2”“Cage 1” Tunnelhouses (horticulture) Year 1 Year 2 Year3 X X Field – Karalee redevelopment

22. © University of Sydney Field - proposed restructure, Horse & Sheep Units Horse Unit Irrigation line

23. © University of Sydney USyd ACRCP Outputs, 5.5 years, 01/07/2007 to 28/02/2013 – Outreach and training Wrote/ distributed/completed: 6 Annual Pathogenicity Survey reports 41 Cereal Rust Updates 75 extension articles for newspapers (incl. Ground Cover) etc 44 media releases including radio interviews. Contributed to 43 grower/ agronomist workshops/ field days Participated in 25 national/ international conferences/ meetings Published 88 peer-reviewed journal articles/ book chapters Completed 13 PhD and 5 Masters students, in rust pathology and genetics

24. © University of Sydney Extension - All Stage / Adult Plant Resistance

25. © University of Sydney

26. Genetic basis of disease response Rust resistance genotypes: Cereal Rust Reports Rust ResponseRust Resistance Genotype Leaf Rust Stem Rust Stripe Eastern States WAWA ptYr17-27 ptLeaf RustStem Rust Stripe Rust AGT Katana MSMRMS-SMR-MS Lr13 Sr30 AGT Scythe MS R-MRMS-S Sr13 Amarok RRSRMR-MS Lr14a, Lr37 Sr38Yr17 Annuello R-MR MS-S Lr24, Lr34 Sr24Yr18 Arrino MS S-VSSS Sr30 C Arrivato MR RR-MR Sr8b, Sr9e, Sr13 Axe MR MSR-MR Sr8b Babbler MSRR-MRMS-S Lr24, Lr34 Sr24Yr18 Banks MR MR-MS Lr13 Sr30 Barham MR-MS MSRMS-S Lr1, Lr20, Lr34, Lr37 Sr9b,Sr15, Sr38 Yr17, Yr18

27. © University of Sydney

28. Current research activities – Rust pathogens DNA marker development – SSRs now available for: - P. graminis - all formae speciales - P. striiformis – all formae speciales - P. triticina - P. hordei Old “104” “WA” pt. USA BGYR BYRPsdPsp e.g. P. striiformis [Jordan Bailey]:

29. © University of Sydney P Stem rust race Ug99 - lecture overview Pgt sequencing initiative - have 86 isolates to sequence - sequenced 61 so far, total cost $60k - dendrogram based on 3.042 million polymorphic SNP loci

30. © University of Sydney P Stem rust race Ug99 - lecture overview Pgt sequencing initiative - have 86 isolates to sequence - sequenced 61 so far, total cost $60k - dendrogram based on 3.042 million polymorphic SNP loci “Ug99” group

31. © University of Sydney P Stem rust race Ug99 - lecture overview Pgt sequencing initiative - have 86 isolates to sequence - sequenced 61 so far, total cost $60k - dendrogram based on 3.042 million polymorphic SNP loci

32. © University of Sydney P Stem rust race Ug99 - lecture overview Pgt sequencing initiative - have 86 isolates to sequence - sequenced 61 so far, total cost $60k - dendrogram based on 3.042 million polymorphic SNP loci

33. © University of Sydney P Stem rust race Ug99 - lecture overview Pgt sequencing initiative - have 86 isolates to sequence - sequenced 61 so far, total cost $60k - dendrogram based on 3.042 million polymorphic SNP loci - informed selection of isolates for 1 st cohort means a huge amount of information will be extracted from this data set

34. © University of Sydney Wheat - APR and ASR to stripe rust, leaf rust and stem rust; cytogenetics Barley - APR and ASR to leaf rust, ASR to stem rust; cytogenetics Oats - APR and ASR to crown rust and stem rust Triticale - APR and ASR to stripe rust, leaf rust and stem rust Non-host / near-host resistance in cereals to rust diseases Current research activities– Gene discovery & characterisation

35. © University of Sydney Backcrossing stripe rust resistance genes into Avocet; 41 gene combination lines produced so far: Current research activities– Gene discovery & characterisation Yr1/Yr6 Yr1/Yr9 Yr1/Yr10 Yr1/Yr17 Yr1/Yr26 Yr1/Yr27 Yr1/Yr32 Yr1/YrSp Yr5/Yr26 Yr5/Yr27 Yr6/Yr27 Yr6/Yr9 Yr6/Yr17 Yr6/Yr24 Yr6/Yr32 Yr6/YrSp Yr7/Yr17 Yr7/Yr24 Yr7/Yr27 Yr7/Yr32 Yr10/Yr15 Yr10/Yr24 Yr10/Yr26 Yr10/Yr27 Yr15/Yr26 Yr15/Yr24//Yr10 Yr17/Yr27 Yr17/Yr32

36. © University of Sydney The Rust Bust Campaign Belinda’s Rust Bust Presentation

37. © University of Sydney