1. Biological Membranes Fig 10-14 Lipids define space, provide barrier, act as label

2. What else is in biological membranes? Proteins What is a protein? (in general terms) What functions do they perform?

3. Membrane Proteins Transmembrane/Integral or Peripheral What functions would require each category of membrane protein?

4. Cells are stable… CBI 10.1 And yet they do this? How?

5. Fluid Mosaic Model (revised) 1. Length and saturation of fatty acid chains in phospholipids

6. More model 2. Amount of Cholesterol (and cholesterol paradox) 3. Temperature

7. More model 2 If ‘everything can move’ is everything randomly distributed? Lipid rafts Tethering Complex formation Why is this level of organization important for correct cellular function?

8. Questions?

9. Membranes define organelles. How?

10. Cell City

11. Coordinating the parts and the whole Overcoming the barrier  ways to cross a membrane gated transport transmembrane transport vesicular transport  ways to signal without crossing receptors and conformational change

12. Case Study 1: Symptoms: One or more of the following Nervousness Irritability Rapid Heartbeat Increased Sweating Fine tremors of hands or feet Sudden weight loss What else?

13. Graves’ opthalmopathy

14. Pretibial dermopathy

15. Goiter

16. History

17. Cause? Unknown Players?

18. Why use this case study? Compare what should happen with What actually occurs Focus on:Extracellular signaling Intracellular signaling Protein structure and function

19. This week in Cell Bio Lab: Getting a feeling for the organism Mating vs budding yeast what can you tell? microscopy/ plating experimental design Class: Extracellular signaling and cellular receptors