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Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of.

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Presentation on theme: "Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of."— Presentation transcript:

1 Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of the Same Patient by H. Claude Sagi, Megan L. Young, Louis Gerstenfeld, Thomas A. Einhorn, and Paul Tornetta J Bone Joint Surg Am Volume 94(23):2128-2135 December 5, 2012 ©2012 by The Journal of Bone and Joint Surgery, Inc.

2 Fig. 1-A Bone harvesting with use of the Reamer/Irrigator/Aspirator (RIA). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

3 Histological assessment of Reamer/Irrigator/Aspirator (RIA) and iliac crest (IC) bone graft materials (hematoxylin and eosin). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

4 Figs. 3-A and 3-B Initial comparative assessment of gene expression as indicated by a microarray analysis of samples from six patients (identified as 2 and 12-16). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

5 Identification of biological and molecular pathways that showed statistically significant differences in quantitative gene expression between samples obtained with the Reamer/Irrigator/Aspirator (RIA) and samples obtained from the iliac crest. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

6 Comparison of the distribution of biological functions associated with the genes in the three relative expression groupings in Figure 4. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

7 Validation of the microarray analysis by comparison with qRT-PCR (quantitative reverse transcription-polymerase chain reaction) measurements. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94:2128- 2135 ©2012 by The Journal of Bone and Joint Surgery, Inc.

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