1. Genetic Engineering

4. Major Techniques PCR – Polymerase Chain Reaction Restriction Endonucleases and Gel Electrophoresis DNA Profiling/DNA Fingerprinting Gene Transfer: Transformation

5. Polymerase Chain Reaction Used to make millions of copies of select section of DNA When small amount of DNA are found but large amounts are needed for analysis Semen, blood, other tissues, long-dead specimens – DNA from all can be amplified Thermus aquaticus – hot springs bacterium http://www.dnalc.org/ddnalc.org/resources/pcr.ht mlhttp://www.dnalc.org/ddnalc.org/resources/pcr.ht ml

6. Gel Electrophoresis

7. Separate DNA, proteins or other molecules based on charge and size

8. DNA Profiling/Fingerprinting

9. DNA Profiling Uses CSI Paternity CDC – epidemiology: disease transmission Geneology Breeding authenticity

10. Who did it?

11. Gene Transfer and GMOs

12. Organisms that have had genes transferred into them from a different species are GMOs or Transgenic Orgs.

13. Pros and Cons of GMOs Be able to explain the + and – of Bt maize. At least 2 arguments for each case

14. Animal/Plant Cloning Plants = easy….from parts. Animals = difficult Procedure:

15. Therapeutic Cloning Be able to argue for and against TC with at least two points in each case Be able to summarize the aims, outcome (to date) and benefits of the Human Genome Project (THGP)