1. ©2014 Waters Corporation 1 ENHANCED MS WITH THE TURN OF KEY

2. ©2014 Waters Corporation 2 Introducing ionKey/MS What does ionKey/MS™ deliver? 2-40x sensitivity vs. your current MS UPLC ® performance and reliability Standardization and consistency Flexibility

3. ©2014 Waters Corporation 3 In Search of a Step Change in Sensitivity?

4. ©2014 Waters Corporation 4 Maximizing signal Maximizing robustness Xevo ® TQ-S A Step Change in Sensitivity

5. ©2014 Waters Corporation 5 Xevo TQ Xevo TQ-S Enhanced sensitivity >25X increase in peak area >5X increase in signal:noise Relative ion abundance Reserpine (50fg) UPLC/MRM Xevo TQ-S A Step Change in Sensitivity

6. ©2014 Waters Corporation 6 Where Next to Look for Sensitivity?

7. ©2014 Waters Corporation 7 Where to Next? 75µm ID 150µm ID 300µm ID + Very Sensitive Flow rates too low for routine use Very Fast Marginal sensitivity increase 0 Fast and Sensitive Useable routinely

8. ©2014 Waters Corporation 8 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

9. ©2014 Waters Corporation 9 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

10. ©2014 Waters Corporation 10 More Sensitivity ionKey/MS 20X Increase Standard 2.1mm column

11. ©2014 Waters Corporation 11 More Sensitivity ionKey/MSStandard Flow LC-MS ionKey/MS 2 Fold suppression in signal due to Matrix Effects 2.1mm 24 Fold suppression in signal due to Matrix Effects

12. ©2014 Waters Corporation 12 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

13. ©2014 Waters Corporation 13 UPLC Performance You Know

14. ©2014 Waters Corporation 14 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

15. ©2014 Waters Corporation 15 Sensitivity Reproducibility Robustness User Expertise Requirement Applicability to Routine Capillary scale UPLC can be challenging

16. ©2014 Waters Corporation 16 ionKey/MS is Plug and Play ENHANCED MS WITH THE TURN OF KEY

17. ©2014 Waters Corporation 17 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

18. ©2014 Waters Corporation 18 Robust, Reliable Reproducible Retention Time Reproducibility 15 QCs over 500 crashed plasma injections Reproducibility of the same compound over: 1.64% RSD 5 Systems 18 iKeys 5 Users

19. ©2014 Waters Corporation 19 Validated Assay Teriparadite (Forteo ® ) Avg MWT 4117.7

20. ©2014 Waters Corporation 20 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

21. ©2014 Waters Corporation 21 The Future of MS Already over 25 publications and presentations in leading scientific journals and conferences such as: Journal of Chromatography, Bioanalysis Journal, Journal of Proteomics, ASMS, HPLC, EBF CPSA, and more. Already over 25 publications and presentations in leading scientific journals and conferences such as: Journal of Chromatography, Bioanalysis Journal, Journal of Proteomics, ASMS, HPLC, EBF CPSA, and more.

22. ©2014 Waters Corporation 22