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Drosophila Identification of various phenotypes.
Biology 22 Drosophila Identification of various phenotypes.
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Reference for Pictures:
Carolina Drosophila melanogster Manual ( ) Published: Carolina Biological Supply Company
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The life Cycle
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Life Cycle: Drosophila melanogaster
Eggs: contain filaments 2 molting phases “instars” Life cycle is 10 days
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Fruity Fly Egg and Larva
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Fruit Fly Pupa and Adult
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Identification of: Gender Body Color
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Wild Type Versus Mutants
Body Color Wild type: Beige Mutant: Yellow body
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Parental phenotypes Experiment: body color
A= female: wt (beige) B=female: wt (beige) C=male: wt (beige) D=female: mutant (yellow) E=male: mutant (yellow)
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Fruit Fly Body Color
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Sex combs: Male Drosophila
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Abdominal segements 7 segments to female abdominal segments
5 segments to male abdominal segments Male body size is smaller than female But the “last” abdominal segment is larger in male
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Nap Time Triethylamine
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How to anesthetize a fly
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How to use the Napper
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View the Fruit Fly
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You will record class data
After counting the crosses record the data on the class spreadsheet
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3 point test cross: Experiment 2
Eye color Body color Wing length
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3 point test cross for: Beige (bl+) vs. Black (bl) 1. Body Color
♦ Wt vs Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 1. Body Color 2. Wing length 3. Eye Color
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Crosses: Female Males Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw
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There are all sorts of eyes!
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Eyes
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You will have only red eyes (wt)
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Fruit Fly Wings
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Parental phenotypes A= female: wt beige body B=female: wt beige body
C=male: wt beige body D=female: yellow body E=male: yellow body
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Female and Male Fruit Flies
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