1. The immunogenetic mechanism of renal injury George P Lai

2. Basic component of renal immune response Humoral response –Anti-GBM disease –Immune complex disease The cellular response –T cells and macrophages –Particularly in crescent formation and tubulointerstitial disease

3. Adaptive immunity

4. To Establish Relationship A present when B occur (TNF elevated when GN occur) B improved when A removed (TNF knock out model) A transfer will make B occur (Transfer model, transgenic model)

5. Animal Models NTN model (Nephrotoxic nephritis)-Crescentic GN –Rat: WKY (macrophage dependent) –Mouse: C57BL/6 (T cell/Th 1 dependent) –Rabbit EAG model (Experimental autoimmune glomerulonephritis) Thy 1.1 model (mesangial proliferative GN) Heymann’s nephritis—MGN Puromycin induced nephrosis--MCD

6. Main players in GN Macrophage T cells B cells Neutrophil Endothelial cells Mesangial cells

7. The results come from The initiating events The genetic background The immune responses (mechanisms) –Humoral immunity –Cellular immunity The glomerular responses

8. Initiating Events Infectious antigen: streptococcus, virus (HBV, HCV, HIV) –Circulating immune complex –Autoimmune response Non-infectious antigen –Drug, toxin Modified by genetic background –Suspectibility (DRB1 in goodpasture’s disease; DR3 in MGN;  1- antitrypsin deficiency in Wegener’s granulomatosis) –Severity –Response to therapy –Outcome

9. Humoral mechanisms of renal injury Predominantly IgG (Except in IgA nephropathy) –Trapping of circulating immune complex Subendothelial, mesangial areas –In situ formation of immune complex Fixed glomerular antigen(anti-basement membrane antibody) Planted antigen (PSGN, Lupus, Goodpasture, MGN)

10. Immunoglobulin

11. Immunoglobulin classes

12. Injury induced by antibody alone Complement independent Anti-nephrin antibody –A slip-pore diaphragm antigen –Offering insight into glomerular permeability barrier Nephrotoxic nephritis –Cathepsins B and Cathepsins L –Cysteine proteinases inhibitor such as E-64, Ep 475 reduced proteinuria

13. Goodpasture’s syndrome

14. Injury mediated by C5b-9 C5b-9 deposition in –subepithelial area induce proteinuria Activate epithelial cell---overproduction of oxidant, proteases, growth factors such as PDGF –Subendothelial and mesangial area Adhesion molecule Proinflammatory cytokines such as IL-1  Growth factors (TGF-  1) C3 NeF-autoantibody nephritic factor 3 –Autoantibody that stabilize the alternative pathway C3 convertases---membranoproliferative GN type II

15. C5a C5a a chemotaxic factor for neutrophil Adhesion molecule upregulated in endothelial cell and neutrophil Selectin, ICAM-1, VCAM-1 Evidences of neutrophil in glomerular disease –PMN can injure glomeruli and degrade GBM in vitro –PMN are present in glomerular disease –PMN depletion reduces glomerular injury in experimental models such as nephrotoxic nephritis –PMN repletion reconstitutes injury in PMN-depleted rats with nephritis

16. Neutrophil Evidences of neutrophil in glomerular disease –PMN can injure glomeruli and degrade GBM in vitro –PMN are present in glomerular disease –PMN depletion reduces glomerular injury in experimental models such as nephrotoxic nephritis –PMN repletion reconstitutes injury in PMN-depleted rats with nephritis

17. Neutrophil induced glomerular injury Neutrophil induced disease by –Release Oxidants: OH, O 2 -, H 2 O 2 –Proteases: lysozyme, defensins –Lipid mediators: thromboxane A2, Leukotriene B4, –Cytokines: IL-1 TNF, IL-6 –Reactive nitrogen species: NO, peroxynitrite

18. Platelets Evidences of platelet involvement –Platelet activation in MGN, Lupus, MPGN, FSGN, DM nephropathy –Elevated platelet secretory products:  - thromboglobulin, PF4 –Renal localization of platelets: mesangiocapillary glomerulonephritis, FSGN, Lupus nephritis

19. Platelets induced renal injury   -granule : PDGF, TGF- , HGF, EGF Lysosomal enzymes –Proeinases: cathepsin, collagenase, neutral protease, acid hydrolases Lipid and eicosanoids –Phospholipase A2 –Prostaglandin E2 –Thromboxane A2 –Leukotrienes

20. Cellular immunity T cell and macrophages T cell activation and antigen presentation –Antigen presenting cells: dendritic cells, B cells and macrophage –MHC class I—CD8; MHC class II—CD 4 –Costimulatory signals (Signal II) B7.1, B7.2—CD28; LFA-1---ICAM family; CD40—CD40L –Immunological synapse

21. Macrophage and T cell T cellMacrophage

22. T cell receptors

23. Immunologic synapse

24. T helper and cytotoxic cells

25. Cellular immunity T cells are important in transplantation biology and crescentic glomerulonephritis T cell effector functions –Cytotoxic T cell: perforin, FasL –Naïve T cell---Activated Th0 cell (IL12) Activated Th1 cells: IL-2, IFN-  TNF-  (IL-4) Activated Th2 cells: IL-4 IL-5, IL-10, IL-11, humoral immunity

26. Macrophage

27. Cellular immunity Macrophages –Multiple roles in glomerulonephritis Antigen presenting cells Effector cells (activation and proliferation in glomeruli) Profibrotic activity: Tissue factor, procoagulant factors(fibrin) Tissue repairing:phagocytosis, removing apoptotic cells, secreting HGF, VEGF

28. Mediators macrophages secrete Reactive oxygen species Nitric oxide Metaloproteinases Complements Procoagulant activity Thromboxane/eicosanoids IL-1, IL-4, IL-10, IL-12 TNF-  MIF PDGF, TGF- , Fibronectin, FGF-2 PAF

29. Glomerular response to immune injury Four factors are important –Site of immune event happen Subendothelium—attract severe inflammation and decrease GFR such as PSGN and Type IV lupus nephritis Mesangium– mesangial proliferation and matrix deposition such as IgA nephropathy Subepithelium—few systemic reactions; injury occurred through C5-9 deposition and local release of mediators

30. Glomerular response to immune injury The mechanism of deposition –In situ immune complex formation is more damaging than pass trapping circulating immune complex The property of deposited antibody –IgG>IgA>IgM The quantity of IgG deposition beyond mesangium> within mesangium

31. Glomerular intrinsic cells Mesangial cells, Endothelial cells, epithelial cells Mesangial cells: –Best studied among the three –Proliferate in response to injury such as IgA nephropathy, Lupus nephritis –Release proinflammatory mediator Oxidants and proteases Cytokines: MCP-1, growth factor(PDGF), prostaglandins, leukotrienes, endothelin, nitric oxide

32. Glomerular intrinsic cells Glomerular epithelial cells –Important in pathogenesis of minimal change disease, focal sclerosing GN, MGN, HIV related GN –Release mediators such as hydrogen peroxide and matrix components –Don’t proliferate in response to mitogen

33. Glomerular intrinsic cells Endothelial cells –Lease studied –Important in Hemolytic uremic syndrome, Thrombotic thrombocytopenic purpura, HCV related renal disease, cryoglobulinemia, lupus nephritis –Adhesion molecule, angiogenesis, production of pro & anti-coagulant molecules

34. Cellular mediator of interstitial disease Tubularinterstitial injury is the common pathway to end stage renal disease Involved T cell, dendritic cell and macrophage, fibroblast Tubular interstitial nephritis without glomerulonephritis may due to –expression of latent protein from virus-herpex or lentivirus –Lose immune tolerance –Tubular reclamation –Secretion of chemokine and cytokine Tubulointerstitial nephritis after glomerulonephritis –Heavy proteinuria, activate complement, chemokine, cytokine during tubular reclamation

35. Humoral mediator of interstitial nephritis Source: adjacent somatic cell(epithelial cell, dendritic cell) Mediators: prostaglandin, leukotriene nitric oxide endothelin, complement, protease, oxidants Persistence is important (1-2 weeks) Fibrogenesis—TGF- , angiotensinII, PDGF, plasminogen activator inhibitor 1, fibroblast specific protein 1.