1. 1 Barnes August 2007 - Who 16 – 23 Aug 1. Jim Postel, marine technician (UW) 2. Lydie Herfort, chief scientist (OHSU) 3. Mike Malpezzi (UMCES) 4. Tiffany Gregg (OSU) 5. Bill Howe (OHSU) 6. Suzanna Brauer (OHSU) 23 – 30 Aug 1. Jim Postel, marine technician (UW) 2. Lydie Herfort, chief scientist (OHSU) 3. Mike Malpezzi (UMCES) 4. Tiffany Gregg (OSU) 5. Dan Murphy (OHSU) 6. Karen Wegner (OSHU)

2. 2 Tiffany Gregg / Fred Prahl Transport of polycyclic hydrocarbons (PAHs) with suspended particulate matter from the Columbia River to its estuary (River & ETM) Lydie Herfort / Dan Murphy / Bill Howe / Mike Malpezzi / Karen Wegner/ Peter Zuber / Byron Crump/ Holly Simon Microbial community structure & gene expression, environmental variables (River, ETM, limit salinity intrusion) Suzanna Brauer / Brad Tebo Characterising rates of Mn oxidation using 54-Mn & 14C- bicarbonate uptake (ETM) Sampling waterair pump (70L) sediment Shipeck grab Barnes August 2007 - What

3. 3 Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide

4. 4 Beaver Dock Columbia Willamette Confluent Willamette & Columbia Locations of river end-member St Helen 0 PSU 0 / 7 PSU 0 / 20 PSU Aim: good river-end member Sampling surface & bottom water in estuary and surface in river

5. 5 Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide

6. 6 Estuary Bathymetry

7. 7 Tide in Astoria (17-29 Aug) and ETM sampling days Neap tideSpring tide S M N S N S M N S = South Channel M = Mouth Channel N = North Channel = ETM sampling day Sampling surface & bottom water Aim: 1.Role of the ETM as a trap for PAHs 2.Discriminate particle-attached and free-living microbial communities and gene expression 3.Rate of Mn oxydation

8. 8 Plan for Barnes: where River ETM Neap tide Limit salinity intrusion ETM Spring tide

9. 9 Tide and limit of salinity intrusion sampling days S = South Channel N = North Channel = sampling day Sampling surface water Aim: 1.Determine diffusion of microbial populations at the limit of salinity intrusion (0-5 PSU) N S

10. 10 Plan for Barnes: how many samples

11. 11 Tides & day/night

12. 12 Samples to collect = 252 (X6 = total 1512 Sterivex) ETM, surface and Bottom water then separated into Bulk and Free-living bacteria S = surface water B = bottom water

13. 13