1. M3/31EXAM IIChapters 8-12, parts of 2, 3 W4/2Transcription and TranslationChapters 4, 15 M4/7 Regulation of Gene ExpressionChapter 18 W4/9"Molecular" GeneticsChapter 16 M4/14 "Classical" GeneticsChapter 13 W4/16DNA ReplicationChapter 14 M4/21Genomics and ProteomicsChapter 20 W4/23EXAM III Chapters 4,13-16,18 M4/28Molecular DevelopmentChapter 22 W4/30Molecular Development Cumulative Final Exam: Wednesday, May 7 th, 10:45-12:45, room 303 Payson-Smith Amazingly, Ashley found the Protein Synthesis Hippie Video on YouTube: http://youtube.com/watch?v=u9dhO0iCLww

2. TRANSCRIPTION TRANSLATION

12. M3/31EXAM IIChapters 8-12, parts of 2, 3 W4/2Transcription and TranslationChapters 4, 15 M4/7 Regulation of Gene ExpressionChapter 18 W4/9 Molecular Genetics Chapter 16 M4/14 "Classical" (Transmission) Genetics Chapter 13 W4/16DNA ReplicationChapter 14 M4/21Genomics and ProteomicsChapter 20 W4/23EXAM III Chapters 4,13-16,18 M4/28Molecular DevelopmentChapter 22 W4/30Molecular Development

13. You inherited one copy of each of your genes from your mom and one from your dad. The genes from your mom and dad are similar but not identical. For example, you inherited two copies of the LDL receptor gene. They may be identical but there is a very good chance that some of the nucleotide letters are different between the two genes. Each version is called an allele. In a population of organisms of the same species, there can be a variety of versions of each gene. From your Mr. Spock handout:

14. Different alleles of the LDL receptor gene can have differences in their coding region that lead to differences in their primary amino acid sequence that lead to differences in their structure that lead to differences in their function. The differences don’t change the basic function of the LDL receptor but, for example, one allele might code for a receptor that is somewhat damaged and only works half as well as the protein coded from a different allele. Wild-type is what we call the allele that has The typical or “average” activity level for the gene. We could say wild-type is fully functional and equivalent to 100% activity. More from your Mr. Spock handout:

15. Any change in the DNA sequence within the gene is a mutation and produces a new allele and a mutant genotype and that could affect the phenoptype. If the mutant allele causes a decrease in gene function it is said to be a loss of function allele. Maybe the allele only works 50% as well as wild-type, or maybe only 15%, or maybe it doesn’t work at all (0% this complete loss of function is given a special name, a null allele). If the allele causes an increase above wild-type it is said to be a gain of function allele. More from your Mr. Spock handout:

16. A A- B+ B C+ C C- D+ D- F D _____ 104 * 102 100 * 98 96 * 94 92 * 90 * 88 * 86 ** 84 ** 82 ** 80 ** 78 76 ** 74 **** 72 ** 70 ** 68 **** 66 **** 64 *** 62 *** MEAN = 63 60 ** 58 56 * 54 ** 52 * 50 ** 48 *** 46 * 44 ***** 42 *** 40 * 38 ** 36 **