1. Supplementary Figure 1 0 -2 -3 -4 0 -2 -3 -4 SU11274 PHA665752 SGX523 827 + EGF/bFGF 827 + HGF SU11274 PHA665752 SGX523 A. B. 100806040200 50403020100 100806040200 05101520 050100150200 050100150200 number of plated cells 131 + EGF/bFGF 131 + HGF 0 -2 -3 -4 0 -2 -3 -4 number of plated cells SU11274 PHA665752 SGX523 SU11274 PHA665752 SGX523 050100150200 050100150200 050100150200 5004003002001000 050100150200 0 20406080100 Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 )

2. 2.08%31.8%25.4% 2.83% 9.00%7.53% Isotype-APC CD133-APC 464T +EGF/bFGF 464T +EGF/bFGF -SU11274 464T +EGF/bFGF +SU11274 464T +HGF 464T +HGF -SU11274 464T +HGF +SU11274 Supplementary Figure 2

3. GFPABCMergeMerge + DAPI Supplementary Figure 3 Field 1 Field 2 Field 3 GFPABCMergeMerge + DAPI GFPABCMergeMerge + DAPI

4. mCMV backbone reporter mCMV-TCF/LEF reporter 464T Relative Luciferase Unit Wnt3a (ng/ml) 02550 Supplementary Figure 4

5. Supplementary table 1 Symbol Name Fold increase in 464T MET high/+ cells Fold increase in 578T MET high/+ cells References CD44 CD44 antigen 3.5912.22 Am J Pathol, 1999, 154(2):515 MET MET protooncogene 1.9919.65 Cancer Res, 2002, 62(18):5126 PLAUR Plasminogen activator receptor, urokinase-type 2.022.74 Proc Natl Acad Sci USA, 1999, 96(4):1603 CCND1 Cyclin D1 2.411.82 Nature, 1999, 398(6726):422 NRCAM Neuronal cell adhesion molecule 2.281.19 Genes Dev, 2002, 16(16)2058 FOSL1 Fos-like antigen 1 1.551.42 Proc Natl Acad Sci USA, 1999, 96(4):1603 JUN v-jun avian sarcoma virus 17 oncogene homolog 1.101.90 Proc Natl Acad Sci USA, 1999, 96(4):1603 L1CAM L1 cell adhesion molecule 1.121.76 J Cell Biol, 2005, 168(4):633 JAG1 Jagged 1 1.151.50 Proc Natl Acad Sci USA, 2009, 106(4)633 CLDN1 Claudin 1 1.281.21 Oncol Res, 2001, 12(11-12):469 TCF7 Transcription factor, T cell-specific 1 1.381.11 Science, 1999, 285(5435):1923 MYC Oncogene myc 1.081.37 Science, 1998, 281(5382):1509 BIRC5 Baculoviral IAP repeat-containing protein 5 (Survivin) 1.21.00 Cancer Res, 2001, 61(24)8664 LEF1 Lymphoid enhancer-binding factor 1 0.912.85 J Biol Chem, 2002, 277(36)33398 BMP4 Bone morphogenetic protein 4 1.410.86 Cancer Res, 2002, 62(10)2744 FGF18 Fibroblast growth factor 18 1.300.92 Cancer Res, 2003, 63(19)6116 GAST Gastrin 1.450.77 J Clin Invest, 2000, 106(4)533 Expression levels of WNT target genes in MET high/+ cells