1. Biuret Test (Write in glossary):
A simple biochemical reaction to detect the presence of protein. A purple colour shows that protein is present, a blue colour shows that protein is absent.

2. Protein Assay Purpose and goals: To pipette accurately To plot results
To analyse results

3. Serial Dilution
Water (cm3)
Protein (cm3)
Absorbance (%) 5 1 4 2 3

4. Quick Questions What was the independent variable?
What was the dependent variable?
What were the control variables?

5. Quick Questions Independent variable = conc. of protein
Dependent variable = absorbance
Control variables = drops of biuret A B, total volume of solution, time