1. Avena genetic resources for quality in human consumption (AVEQ)
Partner 12 IHAR -PIB
Laboratory of Quality Evaluation of Plant Materials
Institute of Plant Breeding and Acclimatization - National Research Institute,
Radzików, PL , Poland
AVEQ Final Meeting – Bucharest October 19-21, 2010

2. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
The objective: Characterise carbohydrates - starch and dietary fibre in genetic resources of Avena.
AVEQ Final Meeting – Bucharest October 19-21, 2010

3. WP 7. Task 1, Sub-task 4 . Analysis of starch - selected method
-amylase/amyloglucosidase method:
ICC Standard no. 128/1and168
or AACC 76-13
or AOAC
AVEQ Final Meeting – Bucharest October 19-21, 2010

4. WP 7. Task 1, Sub-task 4 . Analysis of dietary fibre - selected method
Enzymatic-Chemical Uppsala Method (AOAC or AACC 32-25)
Gas chromatographic-colorimetric-gravimetric procedure
TDF = neutral sugar residues + uronic acids + Klason lignin
Principle
1. Measurement of NSP as neutral sugar residues by GLC
(Englyst and Cummings procedure, 1988)
starch removal by specific enzymes
precipitation of soluble polymers by ethanol
hydrolysis of polysaccharides to monosaccharides with H2SO4
reduction and derivatisation
2. Measurement of uronic acid by colorimetry (Scott method, 1979)
3. Measurement of Klason lignin by gravimetric method as the sulphuric acid resistant residue (Theander et al.,1995)

5. Dietary Fibre Constituents
I. Non-starch polysaccharides & oligosaccharides
Cellulose
Non-cellulosic polysaccharides
Polyfructoses
inulin
oligofructans
Galactooligosugars
Hemicelluloses
arabinoxylans
arabinogalactans
β-glucans
Pectins
Gums
Mucilages
IV. Substances associated with NSP & lignin complex in plants
II. Analogous Carbohydrates
Waxes
Phytates
Cutin
Suberin Saponins
Tannins
Resistant starch
Synthesized carbohydr.
Indigestible dextrins
III. Lignins
AVEQ Final Meeting – Bucharest October 19-21, 2010

6. Material analysed from the year 2008
WP 7. Task 1, Sub-task 4 .
Analyses for starch and dietary fibre
Material analysed from the year 2008
- 60 samples of selected accessions from trial in Bulgaria
- 61 samples of selected accessions from trial in Estonia
- 2 samples of wild accessions from trial in Estonia
- 6 samples of wild accessions from trial in Poland
- 53 samples of standards from trial in Estonia
Total no of accessions: 182
AVEQ Final Meeting – Bucharest October 19-21, 2010

7. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
I set - selected 60 accessions from Bulgaria trial
Range – 64.1%; avg – 56.3%; CV – 4.3%

8. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
I set - selected 60 accessions from Bulgaria trial
TDF - range 9.4–18.2% avg %; CV - 10%
5.0
2.6
6.1
14.1
NSP - range 6.1–14.1%
avg %; CV - 13%
Uronic acids - range 0.14–0.32%
avg – 0.23%; CV - 18%
Lignin - range 2.6–5.0%
avg - 3.3%; CV - 13%

9. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
II set - selected 61 accessions from Estonia trial
Range 48.9 – 64.9%; avg – 58.7%; CV – 5.9%

10. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
II set - selected 61 accessions from Estonia trial
TDF - range 9.0–15.8% avg %; CV - 12%
4.4
1.8
11.8
6.5
NSP - range 6.5–11.8%
avg %; CV - 13%
Uronic acids - range 0.12–0.24%
avg – 0.17%; CV - 16%
Lignin - range 1.8–4.4%
avg - 2.8%; CV - 22%

11. Analyses for starch and dietary fibre
WP 7. Task 1, Sub-task 4 .
Analyses for starch and dietary fibre
II set - selected 61 accessions from Estonia trial plus 2 wild Avena samples
TDF - range 9.0–15.8% avg %; CV - 12%
NSP - range 6.5–11.8%
avg %; CV - 13%
Uronic acids - range 0.12–0.24%
avg – 0.17%; CV - 16%
Lignin - range 1.8–4.4%
avg - 2.8%; CV - 22%

12. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
Polish wild species
Accession no
NSP UA
Lignin
TDF
Starch
AVE 1426
11.3
0.29
4.5
16.1
47.8
AVE 1758
10.3
0.27
4.6
15.1
48.0
AVE 2671
11.2
0.32
4.8
16.3
46.5
AVE 2804
0.30
16.0
48.3
AVE 2781
14.0
5.0
19.3
43.9
PL 52345
4.9
20.3
40.8
AVEQ Final Meeting – Bucharest October 19-21, 2010

13. Analyses for starch and dietary fibre - standards
WP 7. Task 1, Sub-task 4 .
Analyses for starch and dietary fibre - standards
Variety
Origin
Starch [DM%] SD CV
Argentina
ITA
58.8
0.4
0.7
Auteuil
FRA
59.2
1.2
Belinda
SWE
60.7
0.8
1.3
Evora
59.7
Mina
BGR
57.1
0.6
Ivory
DEU
63.5
0.2
Jaak
EST
60.5
1.5
2.6
Saul
CZE
63.0
2.1
Krezus
POL
62.7
3.3
Genziana
59.5
1.8
3.1
Mures
ROM
59.8
3.4
5.7
Mean value 
60.4
2.0

14. WP 7. Task 1, Sub-task 4 . Relationship between NSP on TDF content
[% DM]
NSP [% DM]
AVEQ Final Meeting – Bucharest October 19-21, 2010

15. Analyses for starch and dietary fibre
WP 7. Task 1, Sub-task 4 .
Analyses for starch and dietary fibre
Plans for the next four months:
Additional standard samples (53 samples of 11 cultivars from field experiment in Estonia) at present are analyzing for dietary fibre and they should be completed within 10 day from now.
Analyses for starch and TDF (NSP only) in 70 selected accessions from trial in Poland (2008) and France, Sweden and Italy (2009).
AVEQ Final Meeting – Bucharest October 19-21, 2010

16. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre
This work has been carried out by :
Anna Fraś
Olga Jablonka
Danuta Boros
With appreciable help of two technicians:
Magdalena Bodzon
Monika Skonieczna
AVEQ Final Meeting – Bucharest October 19-21, 2010