1. SALMONELLA TYPHIMURIUM- GETTING TO THE BOTTOM OF IT G. Doran, N. DeLappe, C. O Hare, G. Corbett – Feeney, M. Cormican National Salmonella Reference Laboratory, Galway

2. INTRODUCTION  S. TYPHIMURIUM COMMON IN EUROPE, USA  POULTRY, CATTLE & PIGS MANY FOODS  MULTIDRUG RESISTANT ISOLATES

3. INTRODUCTION -2  MULTI DRUG RESISTANT DT104  INCIDENCE INCREASING  ACSSuT – CHROMOSOMALLY CARRIED  TRIMETHOPRIM, NALIDIXIC ACID RESISTANCE   SUSCEPTIBILITY TO CIPROFLOXACIN

4. TYPING METHODS  PHAGE TYPING  ANTIMICROBIAL SUSCEPTIBILITY  PLASMID PROFILING  PFGE

5. PRINCIPLES OF PFGE CATTTTAC GTAAAATG

6. PRINCIPLES OF PFGE CATGTTAC GTACAATG NO CUT

7. PULSENET  STANDARDISED PFGE  CDC USA  E. coli, Salmonella, Listeria, Shigella  CENTRAL DATABASE

8. PFGE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

9. PLASMID PROFILE 1 2 3 4 5 C C C C 6 7 8 9 10 Size (MDa) 125  26  10  2 

10. S. TYPHIMURIUM 2001  303 ISOLATES  ANIMAL AND HUMAN ORIGIN  PHAGE TYPING (N = 303)  ANTIMICROBIAL SUSCEPTIBILITY (N = 303)  PGFE TYPING (N = 163)  PLASMID PROFILING (N = 36)

12. ANTIMICROBIAL SUSCEPTIBILITY

13. PLASMID PROFILING  36 ISOLATES  MODIFIED METHOD OF KADO AND LIU  PLASMIDS 1.5 - 125 MDA  60MDA PLASMID IN DT104, MDR DT120 AND DT12

14. A B C D E F G H MDRPATTERNPHAGE TYPE DT104, DT104b, MDR DT12 &120 88% MDR 32 ISOLATES FULLY SENS DT12 DT120

15. PHAGE TYPE U310  EMERGED IN 2001  PIGS, HUMANS  15 ISOLATES  MINOR DIFFERENCES ON PFGE

16. DISCUSSION  S. TYPHIMURIUM –  HUMANS & ANIMALS  MDR - INCREASED  DT104 & DT104b  MAJORITY MDR, CLONAL

17. DISCUSSION - 2 DT104, DT104b PFGE U302, RDNC P11 MDR DT12, DT120 P3, P5, P7, P14DT193 P18, P20 P23, P27

18. CONCLUSIONS  PHENOTYPIC & GENOTYPIC METHODS NECESSARY  HIGH INCIDENCE OF MDR IN IRELAND  CONTINUOUS MONITORING OF S. TYPHIMURIUM NECESSARY

19. OTHER RESEARCH  FLUROQUINOLONE RESISTANCE IN S. ENTERITIDIS – DONNA KILMARTIN