1. Sampling Methods

2. Sampling Purpose –Detect and quantify –Identify sources/amplification –Assess potential virulence –Assess effectiveness of controls

3. Cladosporium

4. Steps in Sampling Selection of a laboratory Selection of analyts Selection of methods Sensitivity of method Field sampling QC Reporting and lab support

5. Approaches to Sampling Qualitative - Type Quantitative - Concentration

6. Overview of Microbial Sample Collection Methods (Liquid Impinger)

7. Overview of Microbial Sample Collection Methods (Fungal Spore Sampling & Spore Traps)

8. Overview of Microbial Sample Collection Methods (Impactor Samplers)

10. Overview of Microbial Sample Collection Methods (Sedimentation Settling Plates)

11. Overview of Microbial Sample Collection Methods (Airborne Particulates & Bulk Samples)

12. Overview of Microbial Sample Collection Methods (Dust & Tape Lifts)

13. Tape Lift Sampling

14. Overview of Microbial Sample Collection Methods (Swab/Wipe & Surface Contact Plate)

15. Overview of Microbial Sample Collection Methods (Water Sample & Endotoxins in air)

16. Overview of Microbial Sample Collection Methods (Mycotoxins in Air)

17. Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1,3-D-Glucans in Air)

19. Microbial By Products (Ergosterol in Air) Notes: *Can also analyze for in bulk, surface and water samples. CFU-colony forming units M3-cubic metres of air Mg-milligrams Ng-nanograms Ppm-parts per million Ml-milliliter G-gram **References used to general Table 1 above are listed below 1-8

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21. Air Pump Rotameter Biosis Flow Valve Plate Sampling

23. Stacked Plate Samplers

24. Impinger Sampling Impingement –Liquid media

25. Impinger Sampling Types of Impingers –AGI-30 –AGI-4

26. Impinger Sampling

27. Air Sampling Types of Filtration –Membrane filter cassette

28. Passive Sampling Deposition –Gravity on agar

29. Passive Sampling Types of gravitational –Settling plates

35. Air Sampling (Spore Trap) Collects “everything” –Viable,non-viable,pollen, Unable to differentiate

36. Carpet Sampling Collect spores in the carpet pile using air pump Identifies type of mold Looking for uncommon types Provides historical information

38. Carpet Sampling

39. Data Interpretation Concentration inside versus outside. Mold types inside versus outside. Presence or absence of a specific mold. Location of unknown source. Change in concentration following remediation. Is a “snap shot” in time.

41. Stachybotris

42. Aspergillus

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45. References