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Published byJason Brooks Modified over 9 years ago
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Gel pictures of cloning lab
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Section1 Gel1 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)
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Section1 Gel2 M 1 2 Group1Group2 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette) 5 Kb 3 Kb 1.6 Kb
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Section1 Gel3 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)
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Section1 “control gel” M 1 2 3 4 5 6 M: DNA 1Kb ladder; 1: Purified cut vector (it did not work. But the band should be in the position of red bar); 2:Cut vector (not purified); 3:uncut vector (labeled as PBS); 4: Purified insert (CAT cassette); 5: Cut insert plasmid (not purified); 6: uncut insert plasmid (labeled as CAT) 5 kb 3 kb 1.6 kb
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Section2 Gel1 M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb
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Section2 Gel2 M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb
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Section2 Gel3 M 1 2 Group1 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb
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