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Pathways that Harvest and Store Chemical Energy 6.

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Presentation on theme: "Pathways that Harvest and Store Chemical Energy 6."— Presentation transcript:

1 Pathways that Harvest and Store Chemical Energy 6

2 Concept 6.1 ATP, Reduced Coenzymes, and Chemiosmosis Play Important Roles in Biological Energy Metabolism Five principles governing metabolic pathways: 1. Chemical transformations occur in a series of intermediate reactions that form a metabolic pathway. 2. Each reaction is catalyzed by a specific enzyme. 3. Most metabolic pathways are similar in all organisms.

3 Concept 6.1 ATP, Reduced Coenzymes, and Chemiosmosis Play Important Roles in Biological Energy Metabolism 4. In eukaryotes, many metabolic pathways occur inside specific organelles. 5. Each metabolic pathway is controlled by enzymes that can be inhibited or activated.

4 What are the energy processing strategies that autotrophs use to obtain free energy? Use energy from the environment to convert inorganic molecules into high energy organic compounds They are the producers for all food chains on Earth! Examples: photosynthetic and chemosynthetic

5 How do photosynthetic organisms obtain free energy? Use visible light energy to convert water and CO2 in to organic compounds and oxygen gas Ex: ALL plants, phytoplankton (cyanobacteria and protists)

6 Are plants the only photosynthetic organisms? NO! Most of the oxygen in the atmosphere was produced by cyanobacteria and photosynthetic protists

7 Photosynthesis Reaction

8 Water and CO2 are both required for photosynthesis...How do they get to the sites of photosynthesis?

9 Photosynthesis is a two part process

10 Photosynthesis is described as an endergonic redox process Why endergonic? What substance is oxidized? To what is it oxidized? What substance is reduced? To what is it reduced?

11 What is light? Light is energy! Photons of specific wavelengths of light are used in the light reactions

12 Absorption Spectrum

13 Questions 9 and 10. What are the major photosynthetic pigments? Is this the only photosynthetic pigment? chlorophyll a and chlorophyll b PROTEINS! Accessory pigments—additional pigments that can absorb other wavelengths of light energy

14 Why are plants green? Chloroplasts cannot absorb all wavelengths of light equally and they reflect green

15 The Chloroplast Chloroplasts separate the light reactions from carbon fixation - compartmentalization The light reactions - thylakoid membranes Calvin Cycle - stroma

16 Figure 6.15 An Overview of Photosynthesis

17 How do photosynthetic prokaryotic organisms carry out photosynthesis? Internal folding of the plasma membrane and presence of chlorophyll provides a specialized site for photosynthesis to occur

18 Overview of the Light Reactions Occurs: in photosystems (membrane proteins and pigment proteins) in the thylakoid membranes of chloroplasts Uses: Water, light, NADP+, and ADP Produces: O2 (waste), NADPH, and ATP

19 Figure 6.18 The Molecular Structure of Chlorophyll (Part 1)

20 Figure 6.18 The Molecular Structure of Chlorophyll (Part 2)

21 79 What happens when chlorophyll absorbs light energy?

22 Chlorophyll molecules in photosystems produce high energy electrons when exposed to photons Excited Electrons move through electron transport chains between photosystems This releases free energy used to pump protons across the thylakoid membrane and into the lumen of the thylakoid

23 Non-cyclic electron flow

24 Figure 6.19 Noncyclic Electron Transport Uses Two Photosystems

25 What is the role of water in the light reactions? Water is decomposed (photolysis) at PSII to supply chlorophyll with replacement electrons This produces waste O2

26 How is ATP produced in non-cyclic electron flow? Chemiosmosis! The high concentration of H+ in the thylakoid space passively diffuse through ATP synthase enzyme

27 How is NADPH produced? Why is it considered a “final electron acceptor” Electrons in the ETC are used to reduce NADP+ to NADPH NADPH “accepts” the electrons at the end of the ETC

28 Figure 6.20 Cyclic Electron Transport Traps Light Energy as ATP

29 Summary of the Light Dependent Reactions: Location? Reactants (4)? Products (4)? **The ATP and NADPH produced are needed for the light independent reactions to take place**

30 Overview of the Calvin Cycle Occurs: in the stroma of the chloroplast Uses: CO2, NADPH, and ATP Produces: Organic Molecules (G3P), NADP+, and ADP

31 How can we describe the process of carbon fixation? The ATP and NADPH produced during the L.D. reactions will be used to drive the incorporation of CO2 in to an organic sugar building block called G3P

32 Figure 6.22 RuBP Is the Carbon Dioxide Acceptor

33 What is the first step of the Calvin Cycle? What is the name of the enzyme that catalyzes this reaction? This is carbon fixation. Carbon dioxide is incorporated into the Calvin Cycle by the enzyme RuBisCo. This reaction turns a 5-Carbon RuBP molecule into two 3-Carbon PGA molecules

34 How are ATP and NADPH used in the Calvin Cycle? The energy in ATP and the electrons in NADPH are used to reduce the PGA molecules into 3- Carbon G3P molecules. A total of 12 G3P molecules are produced

35 G3P is the 3-Carbon organic compound produced during the calvin cycle. What are the possible fates of the G3P molecules produced? 10 of the 12 molecules produced are used to regenerate RuBP…Why? 2 G3P molecules exit the stroma and can be combined to… Make sugars and be used in C.R. Excess glucose stored as starch Make lipids, amino acids, or nucleotides

36 Why is photosynthesis the most important biological reaction on Earth? The C—H bonds generated by the Calvin cycle provide almost all the energy for life on Earth. Autotrophs and Heterotrophs rely on this chemical energy to support their own growth and reproduction!

37 Photosynthesis Determines Global Productivity

38 Summary of the Calvin Cycle: Location: Reactants: Products:


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