1. Jonathan Worrall MCB away day 25.09.13

2. An appraisal of metals used in proteins Systematic survey of 1371 different enzymes with a known 3D structure 47% required metals with 41% requiring metals at their catalytic sites JBIC (2008) 13, 1205-1218 Nature (2009) 460, 823-830

3. Research Interests Microbial biotechnology Apoptosis Cytochrome c/phospholipid complexes Streptomyces lividans Cu Worrall & Vijgenboom 2010, Nat. Prod. Reps. 5, 742. Cuproproteins and enzymes must have a role in triggering development switches

4. R5 80 hours R5 10 μM Cu(II) 80 hours S. lividans R5 140 hours ΔSLI4214 ΔSLI4213 ΔSLI4212 ΔSLI3188  Cuproproteins/enzymes have morphological phenotypes Blundell et al. 2013, Open Biology 3, 120163

5.  SLI3188 S. lividans Morphological Engineering Reengineering of Streptomyces strains as hosts for synthetic biology approaches to create sustainable industrial production platforms

6. Techniques used Molecular biology Recombinant protein over-expression Protein chromatography UV-visible, circular dichroism (CD) spectroscopies Electron paramagnetic resonance (EPR) spectroscopy Nuclear magnetic resonance (NMR) spectroscopy (UEA) X-ray crystallography Isothermal titration calorimetry (ITC) Stopped-flow kinetics Electrophoretic mobility shift assays (EMSA)

7. Collaborations UEA (Cytochrome c) Professor Geoff Moore (NMR) Professor Julea Butt (Electrochemistry) Molecular Biotechnology, Leiden, NL Dr Erik Vijgenboom Essex Dr Mike Hough (X-ray) Dr Dimitri Svistunenko (EPR) Professor Mike Wilson (SF-kinetics)