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Microscope Math Biology 11.

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Presentation on theme: "Microscope Math Biology 11."— Presentation transcript:

1 Microscope Math Biology 11

2 Don’t forget to always use units with numbers!
‘times larger’ or ‘x’ mm µm

3 Total Magnification Review
Formula: Total Magnification = (eyepiece)(objective) Low: (10)(4) = 40x Medium (10)(10) = 100x High (10)(40) = 400x

4 Field of View Diameter (widest distance across) of the circle of light when looking through the microscope Expressed in µm (micrometers)

5 Micrometers (µm) 1 µm = 0.001 mm 1 mm = 1000 µm
To convert: Move decimal 3 places! Example: 7752 µm = 7.752mm

6 Measuring the Field Diameter (Low Power)
Place ruler across center of field of view Line up the middle of a mm mark against one side of field of view Count the number of full mm spaces. Estimate amount of next mm if not complete Record results on board

7 Field Diameter Total Magnification Measurement (mm)
We will use the following field diameter sizes. Record the µm measurements on your sheet. Total Magnification Measurement (mm) Field Diameter (µm) Low (40x) 4.2 4200 Medium (100x) 1.68 1680 High (400x) 0.42 420

8 Estimating Specimen Size
If you know how wide your field of view is you can estimate the size of specimens being viewed Expressed in µm (micrometers)

9 Estimating Specimen Size
Formula: Estimated length = Field Diameter (µm) Fit number

10 What is a “fit number”? NUMBER of times an object FITS across the field of view No units are used!

11 Example How many times can this ball fit across the field of view?
Fit number is 3

12 If we viewed the ball with low power …
Formula: Estimated length = Field of View (µm) Fit number = 4200 µm 3 = 1400 µm Because it was viewed on low power

13 Any Questions? Let’s practice!

14 Diagram Magnification
How many times larger your diagram (or scientific drawing) is than the actual specimen Expressed as “times larger” or “X”

15 Diagram Magnification
Formula: Diagram magnification = Diagram size (µm) Estimated Size

16 What is the “diagram size”?
How large your diagram (or scientific drawing) Expressed in µm How to find? Measure your diagram in mm Multiply by 1000

17 Example Diagram the “specimen” below. Determine the fit number.
Calculate the estimated size.

18 Example (cont.) Determine the diagram size in micrometers.
Calculate the diagram magnification.

19 Any Questions? Let’s practice!


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