1. Module 2 Safety precautions for AFB culture and DST of M. tuberculosis Exercise 1 1

2. What’s wrong? 2

3. Surgical masks are not useful in a laboratory setting: they do not protect against aerosols! All persons in the laboratory should wear gowns or laboratory coats. Answers 3

4. What’s wrong? 4

5. Answer Wear gloves while processing specimens. Wear gowns with narrow cuffs 5

6. What’s wrong? 6

7. Do not use gloves for handling non- contaminated devices – or they will become contaminated! Remove gloves after every interruption of activity. Answers 7

8. What’s wrong? 8

9. If respirators are worn, the manufacturer’s instructions must be followed. A respirator must fit the user’s face perfectly, without leakage. Long-sleeved gowns or laboratory coats must be worn. Answers 9

10. What’s wrong? 10

11. Two technicians are working in a small BSC at the same time. One is wearing a surgical mask, one a respirator. Long-sleeved gowns or laboratory coats must be worn. Answers 11

12. What should be done in case of a spill within the BSC? 12

13. Answer Cover the spill with paper towel and soak thoroughly with an adequate disinfectant, allowing 2 hours for contact. Wipe the surrounding exposed surface and equipment with disinfectant. 13