1. 1 Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling Author: Ash A. Alizadeh, Michael B. Eisen et al. Source: Nature vol.403, 2000, pp.503-511 Speaker: Shu-Fen Chiou( 邱淑芬 )

2. 2 Outline Microarray Introduction Design Experimental results Conclusions

3. 3 Microarray(1/2) ( 圖片來源 : 牛頓雜誌 )

4. 4 Microarray(2/2)

5. 5 Introduction(1/3) Lymphoma ( 淋巴瘤 ) – Hodgkin’lymphoma – Non Hodgkin’s lymphoma DLBCL-40% of patients have prolonged survival CLL FL

6. 6 Introduction(2/3) Immune cell ( 免疫細胞 ) – Lymphocyte ( 淋巴球 ) B cell- 製造抗體已抵抗細菌和毒素 T cell- 攻擊已被病毒控制的體細胞或癌細胞 – Monocyte ( 單核白血球 )

7. 7 Introduction(3/3) Lymphocyte ( 淋巴球 ) – Resting ( 等待 ) – Activation ( 活化 ) – Transform ( 轉型 ) plasma cell helper macrophage – Maturation ( 成熟 )

8. 8Design(1/4) Using 128 Lymphchip 18432 genes 96 normal and malignant lymphocyte – 42 DLBCL (Diffuse Large B-cell Lymphoma) – 11 CLL (Chronic Lymphocytic Leukemia ) – 9 FL (Follicular Lymphoma)(CD19+) – 34 other ( 類似正常人 )

9. 9 Design(2/4) 34 other – 2 Germinal Center B (B 細胞生發中心 ) – 4 Resting B Cell – 10 Activated B Cell(CD40L,IL-4) – 10 Transformed Cell – 6 Resting /Activated T Cell(CD4+,CD4+I+P) – 2 Lymph Node ( 淋巴結細胞 -B 細胞聚集的地 方 )

10. 10Design(3/4) No.47

11. 11 Design(4/4)

12. 12 Experimental results(1/6)

13. 13 Experimental results(2/6)

14. 14 Experimental results (3/6)

15. 15 Experimental results(4/6)

16. 16 Experimental results(5/6)

17. 17 Experimental results(6/6)

18. 18 Conclusions We identified two molecularly distinct forms of DLBCL: – germinal centre B cells ('germinal centre B-like DLBCL') – activation of peripheral blood B cells ('activated B-like DLBCL').