1. The Effect of Exercise on O 2 Consumption and CO 2 Production

2. Effect of Exercise on 0 2 Consumption and CO 2 Production Design Outline Hypothesis: Increased exercise/work will increase CO 2 exhaled IV: Amount of exercise Levels of IV: recline, walking, stepping, high exercise (mostly anaerobic) Trials: 27 27 27 27 DV: Amount of CO 2 exhaled (micromoles) and pulse (bpm) Constants: time exercised, time breathing, amount of H 2 O Focus Questions: What’s the relationship between workload, pulse and CO 2 production? Why do different people produce different CO 2 levels with the same workload?

3. Effect of Exercise on 0 2 Consumption and CO 2 Production Lab Chemistry – CO 2 makes acid CO 2 + H 2 O  H 2 CO 3 (acid) H 2 CO 3  HCO 3 - + H + H + determines pH (  H + =  pH-- more acidic )

4. Effect of Exercise on 0 2 Consumption and CO 2 Production Lab Chemistry– Neutralizing the acid by titration NaOH  Na + + OH - (base) OH - + H +  H 2 O (acids and bases neutralize one another). With an indicator, solution will change color when neutral. Solution is neutral when volume NaOH = volume CO 2. NaOH used is an accurate measure of CO 2 in the solution.

5. Effect of Exercise on 0 2 Consumption and CO 2 Production Lab Protocol – (4) Exercise Levels rest-recline position-lay on floor low exercise -walk down north hall only medium exercise –alternate leg step on chairs high exercise – push ups, jumping jack, air squat (knee squats) 3 minutes each Set-up breathing tube before you exercise. Do each test in order. One partner does all four tests, then the other partner does all four tests.

6. Effect of Exercise on 0 2 Consumption and CO 2 Production Lab Protocol – Collecting CO 2 Breathe normally through plastic tubing for one minute. Plug nose as you breathe through tube. Be careful not to breathe too hard and splash water. Begin breathing immediately after activity. Partner measures pulse for 30 seconds. (record as beats/minute)

7. Effect of Exercise on 0 2 Consumption and CO 2 Production Lab Protocol - Titration NaOH can burn-wear goggles. Measure 100 ml of distilled water with graduated cylinder. Add only 3 drops of indicator! Remember to record starting position of NaOH. Add NaOH slowly until it holds color for one full minute.