1. Enteric defensins are essential regulators of intestinal micorbial ecology Nita Salzman Nature immunolgy 11, 76-83

2. The problem Friendly bacteria ? ??

3. Background Mucous membrane Lumen Friendly bacteria Lamina Propria DC Innate proinflamatory response Il-1, a,B- befensins TL5,9 Cytokines IgA Tcell IL-17

4. Previously DEFA-5 Protection against Salmonella

5. Hypothesis Addition of DEFA5, kills Salmonella directly, therefore providing protection, or, DEFA5 alters intestinal flora which in turn provides protection against the pathogen.

6. Expression

7. Bacterial colonisation mRNA

8. So..? So far, mice expressing functional DEFA5, have a different microbiological ecology. Which is not due to other paneth cell functions.

9. Recipricating data

10. SFB

12. Total numbers

13. Th-17 cells

14. The problem refined Friendly bacteria IL-17 T cells

15. Implications Crohns type diseases Probiotics Allergies Antibiotic treatments!