1. Endoparasites – Ruminants

2. Goals and Objectives Understand the influence of parasitism on production Become familiar with the types of parasites afflicting agricultural animals Understand the public health implications of selected parasites Understand the basic principles of parasite control and treatment

3. Parasitology - Ruminants Economic Losses – Poor ADG – Abortion – Decreased conception rates – Death Public Health – Zoonosis

4. Reasons For Economic Losses -Producer Unaware of parasite damage estimate annual parasite-related losses to the livestock industry at more than $100 millionestimate annual parasite-related losses to the livestock industry at more than $100 million - Timing & Frequency of treatments -Choice of dewormer -Parasites have greatest impact on high producing animals.

5. What is ruminants Parasitism? It is a herd diseaseIt is a herd disease It is a production diseaseIt is a production disease It develops during grazingIt develops during grazing 99% of all pastures contaminated99% of all pastures contaminated

6. Level of Parasitism Related To Age of animalsAge of animals Pasture contamination levelPasture contamination level Stocking rate of animalsStocking rate of animals Grazing environment & WeatherGrazing environment & Weather Immune status of animalsImmune status of animals

7. Deworming strategy -Producers understand importance of internal parasite control for production efficiency. -Deworming is now standard practice on beef and dairy farms. -Must have correct product at the correct time.

9. Definition Types of relationships between organism and host – Commensal ‐ one benefits without harming the other – Mutualism ‐ both participants benefit – Parasitism ‐ one benefits at the expense of the other

10. Definition Endoparasite ‐ internal infection Ectoparasite ‐ external infestation Zoonotic infection ‐ transmission of an infection from animals to humans

11. Definition Life cycle ‐ from the start of one generation to the start of the next – Direct ‐ completion of life cycle requires a single host – Indirect ‐ completion of life cycle requires greater than one host

12. Direct Lifecycle

13. Indirect Lifecycle

14. Definition Definitive Host ‐ where sexual reproduction of parasite occurs Intermediate Host ‐ required to complete a developmental phase in the parasites life ‐ cycle, excluding sexual reproduction Pre ‐ patent Period ‐ time from infection of definitive host to the production of parasite offspring

15. Designing a Program in a Dairy/Meat Operation First determine the approximate level of parasitic contaminationFirst determine the approximate level of parasitic contamination

16. 1.- High Parasite Contamination Level Cows grazing pasture during lactationCows grazing pasture during lactation When rotational grazing is practicedWhen rotational grazing is practiced

17. 2. Moderate Parasite Contamination Level Cows grazing pasture only during dry periodCows grazing pasture only during dry period Cows with access to an exercise lot only (with some grass)Cows with access to an exercise lot only (with some grass)

18. Low Parasite Contamination Level Cows with access to dirt dry lotCows with access to dirt dry lot

19. 4. Extremely Low Parasite Contamination Level Cows in total confinementCows in total confinement Cows on a concrete dry lotCows on a concrete dry lot

20. Summary Strategy For Lactating Cows Parasites have been shown to decrease milk production in early lactationParasites have been shown to decrease milk production in early lactation Identify parasite contamination levels in each dairy operationIdentify parasite contamination levels in each dairy operation Design deworming program based on individuals or herd treatment relative to contamination levelsDesign deworming program based on individuals or herd treatment relative to contamination levels

21. Parasite – Indications Purpose of the tests 1.Direct-aids in the detection of certain protozoan trophozoites. 2.Flotation method-eggs in feces are mixed with flotation solution that will cause them to float to the top. 3.Baerman Funnel Technique-larva migrate to the bottom. Used to detect lungworms in ruminants. 4.Centrifugation technique-will detect parasitic eggs when other techniques do not, specially heavier eggs that will not float. 5.MIF (merthiolate iodine formalin) –satins and preserves protozoans & larvae

22. Modified Wisconsin Sugar Flotation Method Technique Samples can be stored if refrigeratedSamples can be stored if refrigerated Sugar solutionSugar solution – One pound of sugar. – Add to 12 oz(355cc) of hot water. Slides can be refrigerated for reading laterSlides can be refrigerated for reading laterMaterials Sugar solution & dispensing syringeSugar solution & dispensing syringe Tea strainerTea strainer 3/5 oz dixie cups3/5 oz dixie cups Tongue depressorsTongue depressors Taper bottom 15cc tubesTaper bottom 15cc tubes Test tube rackTest tube rack Microscope slides & 22x22 mm cover slipsMicroscope slides & 22x22 mm cover slips

23. Modified Wisconsin Sugar Flotation Method Add 15 - 17 cc sugar solution to sample

24. Modified Wisconsin Sugar Flotation Method Place 3 - 5 grams of fecal material into a 3 oz paper cup (About a thimble full)

25. Modified Wisconsin Sugar Flotation Method Stir solution & fecal sample to an even consistency.

26. Modified Wisconsin Sugar Flotation Method Stir solution & fecal sample to an even consistency.

27. Modified Wisconsin Sugar Flotation Method Use a tongue depressor, press as much material through strainer as possible.

28. Modified Wisconsin Sugar Flotation Method 1. Pour into 15cc taper bottom centrifuge tube. 2. Centrifuge in swinging arm centrifuge at 900 rpm for 5 – 7 minutes.

29. Modified Wisconsin Sugar Flotation Method 1. Place tube in rack and top off with sugar solution to form a meniscus. 2. Place 22x22 mm cover slip on tube and leave in place for 2 - 4 minutes.

30. Modified Wisconsin Sugar Flotation Method Lift cover slip upward & place on slide

31. Modified Wisconsin Sugar Flotation Method Use microscope to scan entire cover slip for egg count

36. Definition Types of parasites – Nematodes (phylum nemathelminthes) ‐ round worms – Cestodes (phylum platyhelminthes) ‐ flat worms – Trematodes (phylum platyhelminthes) ‐ flukes – Protozoa (phylum protozoa) ‐ single ‐ celled eukaryotes

37. Nematodes Adult worms – male and female – range in size from large to microscopic Eggs →Larvae (stage 1 ‐ 4) →Adult – Most have direct life cycles – Most transmitted as infective larvae on pasture GI tract and lungs as adults

38. GI Nematodes ~ 11 Genera, Many Species Sites – abomasum, small intestine, cecum, and large intestine Most ruminants = chronic infections Production losses and clinical disease are proportional to severity of infection

39. GI Nematodes – Hot complex Haemonchus contortus – 1” (25 mm) – Abomasum of small ruminants – feeds on blood – Clinical signs anemia death

40. Clinical signs Haemonchus Calf is in poor condition with ‘bottle jaw’ due to hypoproteinemia and anemia. It is massive direct damage, usually late winter.

41. Adults in the abomasum. Barberpole worm

42. GI Nematodes Ostertagia ostertagi (brown stomach worm) – 1/2” (10 mm) adult worm; abomasum – most serious impact on calves – disrupt gastric acid secretion – Clinical signs diarrhea ill ‐ thrift poor feed conversion

43. Ostertagia ostertagi

44. GI Nematodes Trichostrongylus axei – “Bankrupt worm” – Adults ~1/4” (4 ‐ 8 mm); abomasum – Clinical signs –Diarrhea – dehydration –bottle jaw –emaciation

45. Platyhelminthes (flatworms) Hermaphroditic Intermediate host (indirect life cycle) Flattened appearance Tapeworms (Cestodes) Flukes (Trematodes)

46. Tapeworms (cestodes) Adult worms few inches to 15 yards long Segmented worms with attached head (scolex) Ruminants = intermediate host for canids and humans Ruminants eat eggs passed in feces of canids or people

47. Tapeworms (cestodes) Cysts in carcass, pea ‐ size to grape ‐ size (beef measles) People/canids infected by eating encysted beef Carcass condemnation ID, WA feedlots ‐ cattle infected with beef tapeworm of man (Taenia saginata); 10% losses in some feedlots

48. Taenia saginata

49. Liver Flukes (Trematodes) Fasciola hepatica (most common); Fascioloides magna – Live in bile ducts as adults – Aquatic snails = intermediate host – Clinical signs photosensitization reduced ADG hepatitis; clostridial dz →death – Condemned liver at slaughter $millions in losses Eggs: are heavy sedimentation is recommended

51. Protozoa Single ‐ celled eukaryotes Amoeba; Ciliates (not discussed) Apicomplexa – Eimeria, Cryptosporium, Toxoplasma, Neospora Flagellates – Tritrichomonas, Giardia

52. Apicomplexa Intracellular protozoa Coccidia – Sexual reproduction in intestine → oocysts in feces → definitive (direct) host or intermediate (indirect) host

53. Eimeria Direct life ‐ cycle (all ruminants) Invade intestinal epithelium – destruction of epithelial cells – disruption of intestinal function Clinical signs – acute and chronic disease – watery and/or bloody diarrhea – decreased ADG → clinical disease → death – young >> adult

54. Cryptosporidium parvum Apicomplexa Similar to Eimeria Clinical signs – diarrhea 1 ‐ 2 week old calves – disease severity varies Zoonotic: – particularly with immunocompromised host

55. Toxoplasma gondii Indirect life ‐ cycle Cat = definitive host – oocysts shed in cat feces Ruminants = intermediate host – tissue cysts Transmission to developing foetus – abortion Zoonotic

56. Neospora caninum Indirect life ‐ cycle Dog = intermediate host Clinical signs – abortion – neurologic disease in calves born alive

57. Flagellates Mastigophora (flagellates that move with a whip) Extracellular parasites One or more flagella ‐ assist with movement Divide by binary fission Example – Tritrichomonas foetus

58. Tritrichomonas foetus Simple reproduction – binary fission – trophozoite is only stage Venereal disease of cattle (bull = carrier) Clinical signs – early abortion – pyometra – significant $losses due to decreased preg. rate

59. References Large animal clinical procedures for veterinary technicians, Elizabeth A. Hanie, 2006 http://www.caes.uga.edu/publications/pubDetail.cfm?pk_ID=6 196http://www.caes.uga.edu/publications/pubDetail.cfm?pk_ID=6 196 http://courses.cals.uidaho.edu/avs/avs471/Lectures/Lectures% 202010/Lecture%20Parasites%20notes.pdfhttp://courses.cals.uidaho.edu/avs/avs471/Lectures/Lectures% 202010/Lecture%20Parasites%20notes.pdf http://cal.vet.upenn.edu/projects/dxendopar/parasitepages/trem atodes/Fhepatica.htmhttp://cal.vet.upenn.edu/projects/dxendopar/parasitepages/trem atodes/Fhepatica.htm http://cal.vet.upenn.edu/projects/dxendopar/index.html#fecal http://www.sheepandgoat.com/HairSheepWorkshop/parasitism.html