1. Detection of Ziram by LC/APCI/MS Hiroshi MORIWAKI
Osaka City Institute of Public Health &
Environmental Sciences

2. Ziram use toxicity pesticide antifouling agent endocrine disruptor
((CH3)2NCS.S)2Zn
M.W
m.p. 240
Log Pow 1.09
endocrine disruptor
mutagen
carcinogen

3. Analysis of Ziram × Conventional method
Direct detection by GC/MS　→　×
Conventional method
Decomposition of the ligand to CS2 ×
Chemical speciation of dithiocarbamate pesticides
LC/MS ( Direct detection of ziram )

4. Ion mode Ionization Positive ion mode Negative ion mode ESI/MS × ×
APCI/MS ×
Corona current<0.6mA × ○
Corona current>0.6mA

5. Mass spectrum B) A)

6. Corona current vs. peak intensity

7. Cone voltage vs. peak intensity

8. Conditions of APCI/MS

9. Column separation

10. Mass chromatogram

11. Calibration curve

12. Summary Next step ・ Direct detection of Ziram by LC/APCI/MS
・ Corona current > 0.6mA
・ LC separation: GPC column
・ Instrumental detection limit: 2mg/L
Next step
・ Development of the pretreatment
・ Use of LC/MS/MS
ref. H. Moriwaki, Y. Yamaguchi and M. Fukushima:
Rapid Commun. Mass Spectro., in press (2001).